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Lazarus Agus
Pusat Penelitian Biologi-LIPI, Jln. Raya Jakarta-Bogor Km 46, Cibinong 16911
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Journal : Widyariset

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INDUCTION OF TRIPLOID SANDALWOOD PLANT (Santalum album L.) THROUGH ENDOSPERM CULTURE IN VITRO Lazarus Agus
Widyariset Vol 14, No 2 (2011): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.739 KB) | DOI: 10.14203/widyariset.14.2.2011.393-398

Abstract

Sandalwood (Santalum album L.) is an over exploitation that causes the population decreases drastically. An experiment was carried out to get triploid plant by using young seed culture. Young seeds of sandalwood were peeled and grew on solid Murashige and Skoog (MS) media formulation with or without addition hormones of α-napthaleneacetic acid (NAA), 2,4 dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) or kinetin.(K). The young seed did not form shoot, but its endosperm formed callus. The endosperm produced callus 26.67% on media with addition of BA 1 mg/l, but not on media without any addition of hormone. The best callus production was on media with addition of NAA 2 mg/l and BA 1 mg/l. The best somatic embryos formation was NAA 1 mg/l + BA 1 mg/l and shoot formation was 2,4-D 1 mg/l + K 1 mg/l treatments. The resulted shoots from endosperms are expected to be triploid plants.
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