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All Journal Microbiology Indonesia Annales Bogorienses
IQBAL KUSNANDARSYAH
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Identification and characterization of virulence factor of several Indonesian Xanthomonas oryzae pv. oryzae FATIMAH FATIMAH; APON ZAENAL MUSTOPA; IQBAL KUSNANDARSYAH
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.262 KB) | DOI: 10.5454/mi.8.3.3

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is the bacterial causative agent of leaf blight in rice (Oryza sativa L.), the most serious bacterial disease of rice in many rice growing areas worldwide. This study aimed to identify and characterize several virulence factors of seven Xoo isolates from Yogyakarta, West Java, and West Sumatera. The identification of Xoo using 16S rRNA confirmed high homology to Xanthomonas oryzae pv. oryzae PXO99A and revealed three groups. The first group was Xoo93229, the second group containing Xoo1110, Xoo1122, Xoo1130, Xoo7624 and Xoo8024 as the same cluster with PXO99A and the third group was KACC10331 and MAFF311018. The amounts of exopolysaccharide (EPS) and cellulase produced were varying depending on the Xoo isolates. The EPS were produced more by isolate Xoo1130, Xoo1122 and Xoo8024. All tested isolates revealed similar cellulase activity except for isolate Xoo8024. The pathogenicity assay among the Xoo isolate showed that all tested isolates were virulent except Xoo7624. The in planta assay revealed that the tested isolates have multiplied and continued increasing the population size except for Xoo1110 and Xoo7624. High yield of EPS, cellulase activity, more virulence, and increasing population size revealed from isolate Xoo1130 and Xoo1122.
COMPARATIVE ANALYSIS OF VIRULENCE FACTOR BETWEEN DIFFUSIBLE SIGNAL FACTOR BIOSENSOR AND INDONESIAN Xanthomonas oryzae pv oryzae Fatimah; Iqbal Kusnandarsyah; Apon Zaenal Mustopa; Nurlaili Ekawati
Annales Bogorienses Vol. 25 No. 1 (2021)
Publisher : BRIN

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Abstract

Xanthomonas oryzae pv. Oryzae (Xoo) is the causal agent of bacterial blight in rice (Oryza sativa L.). In the understanding of virulence determinants of three Indonesian Xoo, extracellular polysaccharide (EPS) production, cellulose and diffusible signal factor (DSF) were identified. DSF signaling system in Xoo is involved in the regulation of virulence factor production. In this study the comparative analysis was conducted with the DSF biosensor isolate (Xoo-dsf). The results showed that high yield of EPS and cellulose activity revealed from isolate Xoo1130 and Xoo1122 compare to Xoo93229 while Xoo-dsf giving similar result with Xoo1130. The comparative analysis of Xoo1130 and Xoo-dsf showed that Xoo1130 produced three DSF-family signals (DSF, BDSF and CDSF) similar to the DSF biosensor isolate. It assumed that Xoo1130 uses a similar mechanism in Xoo-dsf to autoregulate the biosynthesis of the DSF-family signals.
Co-Authors APON ZAENAL MUSTOPA Fatimah FATIMAH FATIMAH Nurlaili Ekawati