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All Journal Majalah Obat Tradisional

Sri Mulyani

Faculty of Pharmacy, Universitas Gadjah Mada

Author-ID : 3038047

Health Professions Medicine & Pharmacology Public Health

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IDENTITAS MAKROSKOPI, MIKROSKOPI, KIMIAWI, RIMPANG C. Mangga, C. Zedoaria, DAN K. Rotunda Sri Mulyani; Novia Dwi Nugraheni; Hendri Marinda Sari; A. Zulvita Anani Siswondo
Majalah Obat Tradisional Vol 18, No 2 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Zingiberaceae plants in Indonesia has been widely used as traditional medicine, such as Curcuma mangga, C. zedoaria, and Kaempferia rotunda. These three plants known as the temu putih. This study aims to determine the chemical content, macroscopic and microscopic characteristics, in order to obtain characteristics of each of these plants as an aid for identification. Macroscopic examination is conducted by observing the characteristics of the rhizome in both fresh and dried state, while the microscopic examination is done by observing the microscopic characteristics of the powder and fresh rhizome. Examination of the essensial oil is conducted to determine phytochemical compound by using GC-MS method. Result shown these three specimens, macroscopically have different taste and smell. Microscopically, these three specimens have different cork thickness, secrete cell colors, and different amylum size. As identity compound, for C. mangga, C. zedoaria, and K. rotunda oils are mirsena, germakrona and benzilbenzoat respectively.

PENGARUH LAMA PENYIMPANAN TERHADAP STABILITAS FISIK DAN KIMIA LOTION PENUMBUH RAMBUT EKSTRAK BIJI KEMIRI (Aleurites moluccana L. Willd.) Anas Putro Senu Prasojo; Sri Mulyani; Mufrod Mufrod
Majalah Obat Tradisional Vol 17, No 1 (2012)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Etanolic extract 70% of candlenut which has 5% concentration have been reported to have hair restoring activity. This study aims to investigate the effect of length storage on physical stability and flavonoid content of hair restoring lotion candlenut extract so that produce an acceptable form. Lotion were made in variation of xanthan gum concentration 0.5%; 1%; 1.5%. Flavonoid content were measured by densitometry. Observation result of physical stability such as latched power, viscosity test, and flavonoid content were statistically analyzed. Result of formula orientation showed that lotion which has 1% concentration of xanthan gum was the best which has latched power less than 1 second, viscosity increased weekly, and segregation was not occur. Type of emulsion result was o/w. Densitometry result showed that there were decrease of relative flavonoid ontent before and after storage, but it was not significant (α = 0.58).

ANALISIS FLAVONOID DAN TANNIN DENGAN METODA MIKROSKOPI-MIKROKIMIAWI Sri Mulyani; Toga Laksana
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Studies have been conducted to analyze flavonoids and tannins using microscopy-microchemical methods. Materials used for the analysis of flavonoid were orange peel, sonchi leaves, and orthosiphon leaves, with N. exaltata as negative control. While guajava leaves, guazama leaves, and areca seed were used for the analysis of tannins, with ginger rhizome as negative control. The study was conducted in a way of material sliced crosswise, subsequently given the identification reagent for flavonoids (ammonia, NaOH, AlCl3, sitroborat). On the other hand, using FeCl3, fast blue salt, and prusian blue for tannins. Having examined under a microscope, the area which gave a positive reaction to the reagent used, indicate the test materials contained compounds that were analyze. The results showed that this microscopy-microchemical method can be used to analyze the presence of flavonoids / tannins in the test material. Presence of flavonoid compounds will give yellow color with ammonia, NaOH, AlCl3, sitroborat reagent, while blue to black for the tannins with FeCl3, red with fast blue salt, and blue with prusian blue.

UJI AKTIVITAS REPELAN MINYAK ATSIRI JAHE EMPRIT (Zingiber officinale Roxb. “Cochin Ginger”) DAN JAHE MERAH (Zingiber officinale Roxb. var rubrum) DENGAN BASIS MINYAK WIJEN DAN MINYAK KELAPA TERHADAP NYAMUK Aedes aegypti Sri Mulyani; Reidinda Ratna Puspita Sari; Sitti Rahmah Umniyati
Majalah Obat Tradisional Vol 19, No 2 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Aedes aegypti is a vector for Dengue. The use of synthetic repellent cause several health issue. Zingiber officinale Roxb. “Cochin Ginger” essential oil (A) and Zingiber officinale Roxb. var rubrum essential oil (B) was used as a repellent for Aedes aegypti. Increasing of repellent activity was strived by formulating A and B with sesame oil and coconut oil as a base. The aim of this study was to compare repellent activity between A and B, to know the influence of oily base to repellent activity of A and B, to know the effective concentration of essential oil in oily base as a repellent.Isolation of essential oil was done by water and steam destilation. Quantitative and qualitative assays by GS-MS. Repellent activity was tested by three steps: initiation, effective consentration assay, and effective concentration of repellent activity. Essential oil on certain concentration (100%, 50% in the base, the effective concentration of SLD) and Lemon grass essential oil was applied on the surface of palm hand. The hand was put into 20x20x20 cm3 cage containing 25 female Aedes aegypti. The number of Aedes aegypti lied on the hand was noted to count protective capacity. First bite was counted as protective time. Analyse was done by One-way ANOVA and Post Hoc Test Tukey HSD (Homogeneous subsets) or Kruskal-Wallis and Mann-Whitney Test. The result showed that A and B contain camphene, mirsene, 1,8-sineol, l-linalool, l-borneol, neral, geraniol, sitral, and α-kurkumen (only A) that quantitatively different. Statistic result showed that B has a greater repellent activity than A. Sesame oil base give a better effect on repellent activity of sesame oil compare than coconut oil. The effective concentration of Zingiber officinale Roxb. “Cochin Ginger” essential oil and Zingiber officinale Roxb. var rubrum essential oil in sesame oil and coconut oil was 75, 85, 85 and 90%, respectively.

INSENCE COMBUSTIBLE SEREH, CENGKEH DAN JERUK SEBAGAI PENOLAK NYAMUK AEDES AEGYPTI Sri Mulyani; Budi Mulyaningsih; Anindita Winda Lestari; Fitri Ana Munawaroh; Diescendy Selly Anna
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Mosquitoes are one of the insect vector-borne diseases such as dengue, malaria, filariasis, etc. The use of materials whose got repellent activity such as lemongrass, cloves, and orange leaves are one of the best way to be protected from mosquito bites. Combustible insence known as aromatherapy preparations that can be used at any time, which is commonly used in religious events. This research is intended to make preparations of combustible insence which has dual functions, serve as room fresheners and also as repellent against Aedes aegypti. The research is conducted by making preparations of the active ingredient of lemongrass, cloves, and orange leaves, each with 5 different concentrations. Those preparats are tested on 25 mosquitoes contained in double trap mosquito cages. The number of mosquitoes in box A and B will be calculated at 5, 10, 15, 30, 45, and 60 minutes respectively. Thus, the repellent activity can be determined. Chromatogram profile testing is also being conducted using TAS microdestilation metode with several reagents for identification. Result shown that all preparations have mosquito repellent activity with 40% of effective concentration for lemongrass, 20% for orange leaves, 10% for cloves leaves. Chromatogram profile test shows orange leaves have the most spots for terpenoid and fenolic compound, followed by cloves and lemongrass. Spots as compounds with carbonyl groups (aldehydes and ketones) is only found in lemongrass.

GRANUL MINYAK SERAI DAPUR SEBAGAI LARVASIDA NYAMUK Aedes aegypti Sri Mulyani
Majalah Obat Tradisional Vol 19, No 3 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

One way to prevent the spread of Haemorrhage Dengue Fever is the use of abate. The use of abate as larvicides often complained causing an unpleasant smell, and can cause resistance. Lemongrass oil is reported to have activity as larvicides, and this study aims to make granules of lemongrass oil preparation, as well as determining the value of LC50, LC90 against larvae of Ae. aegypti instar III. The granules of lemongrass oil preparation are made with lactose filler and binder CMC-Na. Larvicidal activity test using 20 larvae, 5 concentration solution to each granules, and contacted for 24 hours. Measurement of total larvae deaths, and data were analyzed using probit analysis from Finney modification, to determine the LC50 and LC90 values. Results shown that lemongrass oil can be made as granules with lactose filler and CMC-NA binder. The LC50 and LC90 values of granules from lemongrass oil are 38,30ppm and 51,57ppm.

Co-Authors A. Zulvita Anani Siswondo Anas Putro Senu Prasojo Anindita Winda Lestari Budi Mulyaningsih Diescendy Selly Anna Fitri Ana Munawaroh Hendri Marinda Sari Mufrod Mufrod Novia Dwi Nugraheni Reidinda Ratna Puspita Sari Sitti Rahmah Umniyati Toga Laksana

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