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All Journal Majalah Obat Tradisional
Machwiyyah L.
Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
Health Professions Medicine & Pharmacology Public Health

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AKTIVITAS PENANGKAPAN RADIKAL 2-2’ DIFENIL-1-PIKRIL HIDRAZIL (DPPH) EKSTRAK ETANOLIK DAUN MENGKUDU (Morinda citrifolia L.), DAN BATANG BROTOWALI (Tinospora crispa L.), FRAKSI AIR SERTA FRAKSI AIR TERHIDROLISIS Tatang Irianti; Andayana Puspitasari; Machwiyyah L.; Rabbani HR.
Majalah Obat Tradisional Vol 20, No 3 (2015)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (988.927 KB) | DOI: 10.22146/tradmedj.8850

Abstract

We have investigated the antiradical activity of ethanolic extract of mengkudu leaves (Morinda citrifolia L.) and brotowali stem (Tinospora crispa L.) using radical scavenging assay or DPPH radical. Thin layer chromatograms were also studied to estimate the group of compounds that have antiradical activity.  The objective of this research was to compare the DPPH antiradical efficiency values of water fraction and acid hydrolyzed water fraction in both extracts. The radical scavenging IC50 value of ethanolic extract, water fraction, and hydrolised water fraction (one and thee hours) of mengkudu leaves were 75,65 µg/mL ; 98,68 µg/mL ; 36,27 µg/mL and 33,36 µg/mL  respectively. The IC50 value for brotowali stem were 33.75 µg/mL, 52.29 μg/mL, 31.12 μg/mL and 18.26 μg/mL.  The highest antiradical activity was the hydrolyzed water fraction three hour namely 200 µg/mL of this fraction was able to inhibit 33.12%  and 18.26 μg/mL DPPH radicals.
Co-Authors Andayana Puspitasari Rabbani HR. Tatang Irianti