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Ritbey Ruga
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AKTIVITAS ANTIINFLAMASI EKSTRAK METANOL DAUN PARE HUTAN (Momordica balsamina Linn.) DALAM MENGHAMBAT DENATURASI PROTEIN Minarti Minarti; Ritbey Ruga; Eva Marliana
PROSIDING SEMINAR KIMIA 2021: SEMINAR NASIONAL KIMIA 2021
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Aktivitas antiinflamasi ekstrak matanol daun pare hutan (Momordica balsamina Linn.) dalam menghambat denaturasi protein telah dilakukan. Penelitian ini bertujuan untuk mengetahui aktivitas antiinflamasi dari ekstrak metanol daun pare hutan (Momordica balsamina Linn.) dengan metode penghambatan denaturasi protein. Protein yang digunakan adalah bovine serum albumin (BSA) 0,2% (w/v) dalam tris buffer saline (TBS). Kontrol positif yang digunakan adalah natrium diklofenak. Hasil uji fitokimia ekstrak metanol daun pare hutan (Momordica balsamina Linn.) mengandung senyawa golongan alkaloid, steroid, fenolik dan flavonoid. Hasil uji aktivitas antiinflamasi ekstrak metanol daun pare hutan (Momordica balsamina Linn.) diperoleh nilai inhibition concentration 50% (IC50) sebesar 127,95 µg/mL. Ekstrak metanol daun pare hutan (Momordica balsamina Linn.) memiliki aktivitas sebagai antiinflamasi sedang. Kata Kunci: Momordica balsamina Linn., Antiinflamasi, Bovine Serum Albumin, Inhibition Concentration 50%.
SINTESIS NANOPARTIKEL PERAK MENGGUNAKAN REDUKTOR ASAM ASKORBAT Yusniar Yusniar; Noor Hindryawati; Ritbey Ruga
PROSIDING SEMINAR KIMIA 2021: SEMINAR NASIONAL KIMIA 2021
Publisher : PROSIDING SEMINAR KIMIA

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Sintesis nanopartikel perak menggunakan reduktor asam askorbat telah dilakukan. Tujuan penelitian ini adalah untuk untuk mengetahui proses sintesis nanopartikel perak dan mengetahui kestabilan nanopartikel perak berdasarkan variasi konsentrasi AgNO3 dengan menggunakan reduktor asam askorbat. Pada sintesis ini dilakukan dengan menggunakan variasi konsentrasi AgNO3 dan variasi reduktor asam askorbat. Karakterisasi yang digunakan dalam penelitian ini adalah Spektrofotometer UV-Vis. Hasil Spektrofotometer UV-Vis menunjukan konsentrasi AgNO3 4×10-4 M dengan asam askorbat 1×10-3 M lebih stabil dibandingkan dengan konsentrasi AgNO3 yang lain. Kata kunci: Nanopartikel perak, asam askorbat dan Spektrofotometer UV-Vis.
Analisis Fitokimia Dan Uji Aktivitas Antibakteri Ekstrak Metanol Daun Suji (Pleomle Angusitolia N.E Brown) Lutfiana Devi Kurnia; Ritbey Ruga; Chairul Saleh
JURNAL KIMIA MULAWARMAN Vol 20 No 1 (2022)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v20i1.1106

Abstract

Phytochemical and antibacterial activity assay of methanol extract from Suji leaves (Pleomele angustifolia) were carried out. The aims of this study are to determine secondary metabolites and antibacterial activity of the methanol extract against Streptococcus sobrinus KCCM 11898 and Streptococcus mutans ATCC 25175 by using agar well diffusion. The results showed that methanol extract of suji leaves contain phenolic, triterpenoid and steroid constituents. With 2%, the methanol extract displayed antibacterial activity against Streptococcus sobrinus and Streptococcus mutans with diameter inhibition zones of 19.0 and 10.3 mm, respectively.
EFEK ANTIBAKTERI EKSTRAK BUNGA PACING PUTIH (Costus speciosus (J. Koenig) Sm.) TERHADAP BAKTERI Escherichia coli Ersa Marina Rahmawati; Eva Marliana; Ritbey Ruga
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

This study was conducted with the aim of identifying the antibacterial activity of crude ethanol extract, ethyl acetate fraction, methanol:water fraction and n-hexane fraction of white pacing flower (Costus speciosus (J. Koenig) Sm.). Sample preparation was carried out using the extraction method, namely maceration with 96% ethanol and obtained a crude extract of ethanol and continued by partition by liquid-liquid extraction method and obtained the n-hexane, the ethyl acetate, and the methanol:water fractions. The antibacterial activity test was carried out on the four samples using the well diffusion method with modification against Escherichia coli ATCC 25922. The antibacterial activity test showed activity in the crude extract of ethanol with an inhibition zone diameter of 7.7 mm. Keyword: white pacing, antibacterial, Escherichia coli
SKRINING AKTIVITAS ANTI Escherichia coli ATCC 25922 EKSTRAK METANOL DARI BEBERAPA TANAMAN RIMPANG Alma Yulvita Pebriana; Eva Marliana; Ritbey Ruga
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Antibacterial activity from methanol extracts of edible rhizomes namely temu putih (Curcuma zedoaria), temu ireng (Curcuma aeruginosis), temu giring (Curcuma heyneana) and temu kunci (Boesenbergia pandurata) against Escherichia coli ATCC 25922 based on diameter of inhibition zone by using agar difusion method was conducted. With 1% of each extracts, methanol extract of temu kunci showed excellence acntibacterial acitivty against E. coli with the diameter of inhibition zone of 20,0 mm and followed by methanol extract temu giring is 19,0 mm. While methanol extract of temu ireng and temu putih revealed strong antibacterial activity with inhibition zones of 10.7 and 10,0 mm, respectively. Keywords: Methanol Extract, Antibacterial, Escherichia coli
POTENSI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL BUNGA PACING PUTIH (Costus speciosus (J. Koenig) Sm.) Madaniah Fathirah; Eva Marliana; Ritbey Ruga
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Inflammation is the basic process by which tissues respond or react to infection, irritation, or other injuries. One of the plants that have the potential as an anti-inflammatory is pacing putih (Costus speciosus (J. Koenig) Sm.). This study aims to determine the anti-inflammatory activity of ethanol extract. The method used was the inhibition of protein denaturation with bovine serum albumin and diclofenac sodium as positive controls. The ethanol extract contains phenolics, flavonoids, and saponins. Anti-inflammatory activity (IC50) of 30.9031 mg/L. The ethanol extract of pacing putih flower has the potential to have anti-inflammatory activity. Keywords: Costus speciosus (J. Koenig) Sm.), Anti-inflammatory, Protein Denaturation
Anti-Inflammatory Effect Of Extract And Fractions Of Gaharu (Aquilaria malaccensis Lamk.) Leaves Nur Hidayah; Ritbey Ruga; Chairul Saleh
JURNAL KIMIA MULAWARMAN Vol 21 No 1 (2023)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v21i1.1022

Abstract

Anti-inflammatory activity test of extract and fraction of gaharu leaf (Aquilaria malaccensis Lamk.) against protein denaturation inhibition in vitro has been carried out. This study aims to determine the percent inhibition of protein denaturation and the strength of anti-inflammatory activity of the concentrated methanol extract, ethyl acetate fraction, and methanol-water fraction of gaharu leaves (Aquilaria malaccensis Lamk.). BSA (Bovine serum albumin) is used as the protein to be heated. Diclofenac sodium was used as the positive control, and the negative control used the appropriate solvent. Based on phytochemical screening tests, methanol extracts are known to contain secondary metabolites, including flavonoids, triterpenoids, steroids, and phenolics. The n-hexane fraction contains triterpenoid and steroid compounds. The ethyl acetate fraction contains flavonoid, triterpenoid, steroid, and phenolic compounds, while the methanol-water fraction contains flavonoid, triterpenoid and phenolic compounds. The results of the anti-inflammatory activity test of crude extract methanol and ethyl acetate fraction showed moderate antiinflammatory potential with IC50 values of 181.58- 168.87 ppm, respectively, while the methanol-water fraction has strong anti-inflammatory potential with IC50 value of 60.84 ppm.
Strategi Peningkatan Kualitas Pembelajaran Kimia bagi Siswa SMA Negeri 1 Muara Badak melalui Praktikum Kimia Rita Hairani; Soerja Koesnarpadi; Teguh Wirawan; Ritbey Ruga; Tri Edi Ersanto
Jurnal Abdimas Mahakam Vol. 8 No. 02 (2024): Juli
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24903/jam.v8i02.2595

Abstract

Tersedianya sarana dan prasarana di tingkat sekolah menengah atas (SMA) untuk melaksanakan praktikum kimia berupa laboratorium serta alat dan bahan praktikum kimia sangatlah penting terhadap peningkatan kualitas pembelajaran bagi siswa. SMA Negeri 1 Muara Badak merupakan salah satu SMA yang berada di kecamatan Muara Badak Kalimantan Timur dan memiliki sarana laboratorium untuk melakukan praktikum. Namun kegiatan praktikum kimia terkendala untuk rutin dilaksanakan yang dikarenakan kurang memadainya alat dan bahan praktikum di sekolah tersebut. Sehubungan dengan kendala yang dihadapi dan sebagai upaya implementasi salah satu poin Tri Dharma Perguruan Tinggi, yaitu pengabdian kepada masyarakat, maka tim dosen Jurusan Kimia FMIPA Universitas Mulawarman pada 5 Juni 2023 berinisiatif untuk melakukan pendampingan praktikum kimia dan juga menyediakan alat dan bahan praktikum kimia yang dapat dilaksanakan di sekolah tersebut. Kegiatan ini juga diparalelkan dengan penyampaian materi mengenai keselamatan dan kesehatan kerja (K3) di laboratorium oleh pihak PT. Global Environment Laboratory (PT. GEL) Samarinda yang merupakan mitra kerjasama. Pada kegiatan ini, pengumpulan data menggunakan instrument berupa angket pre-test dan post-test yang disebarkan ke 30 siswa SMA Negeri 1 Muara Badak. Hasil analisis terhadap jawaban angket pre-test menunjukkan bahwa sekitar 71% siswa setuju bahwa kimia adalah subjek yang menarik dan 72% siswa setuju bahwa kimia berguna untuk memahami fenomena sehari-hari. Selanjutnya, berdasarkan analisis data angket post-test menunjukkan bahwa sebagian besar siswa setuju dengan uraian yang dijabarkan di angket. Secara keseluruhan hasil analisis angket-angket tersebut menunjukkan bahwa kegiatan yang telah dilakukan memiliki dampak yang cukup baik bagi siswa SMA Negeri 1 Muara Badak dalam hal motivasi belajar terhadap pembelajaran kimia melalui metode praktikum.
Co-Authors Alma Yulvita Pebriana Ersa Marina Rahmawati Eva Marliana, Eva Lutfiana Devi Kurnia Madaniah Fathirah Noor Hindryawati Nur Hidayah Rita Hairani Saleh, Chairul Soerja Koesnarpadi Syamsul Arifin Teguh Wirawan Tri Edi Ersanto Yusniar Yusniar