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Eirene Natalia Lawodi
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2. VARIASI GENETIK TANAMAN TOMAT DARI BEBERAPA TEMPAT DI SULAWESI UTARA BERDASARKAN GEN MATK Lawodi, Eirene Natalia
PHARMACON Vol 2, No 4 (2013)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.3098

Abstract

Identification and preservation of genetic diversity of a population is important inconservation. This study aims to determine the MATK gene variation among tomato plantsfrom several places in Sulawesi and compare it with the closest relatives in GenBank, as wellas create a phylogenetic tree. PCR technique was used to amplify matK gene with universalprimers. ‘Kinky’ and “Apple” tomatoes were obtained from Minahasa regency of NorthSulawesi province. Both have an identical sequence of partial matK gene. “Cherry” tomatoobtained from North Minahasa regency of North Sulawesi province has a sequence of partialmatK gene which is identical with “cherry” tomatoes obtained from the district of Poso inCentral Sulawesi province. This study implies that both “cherry” tomatoes are the ancestor ofboth “kinky” and “apple” tomatoes.Key words : Genetic diversity, matK gene, Lycopersicon esculentum, phylogenetic tree