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Mohamad Rusdi Hidayat
Baristand Industri Pontianak
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Biogas Production from Tofu Industrial Wastewater with Effective Microorganisms 4 (EM-4) as Biocatalyst Mohamad Rusdi Hidayat
Biopropal Industri Vol 3, No 1 (2012)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.186 KB) | DOI: 10.36974/jbi.v3i1.735

Abstract

The addition of Effective Microorganims 4 (EM-4) activator against the biogas  production was done to utilize the tofu industrial wastewater as one of the substrate for biogas production so it can increase its economic value. The aim of this research was to determine the optimum EM-4 concentration as a biogas activator using tofu industrial wastewater as a substrate. This research was conducted by adding the EM-4 activator with different concentrations of 0.25%, 0.50%, 0.75%, and 1% into the 20 litre of tofu wastewater substrate. The process of biogas formation was observed through a pressure reading on pressure gauge tools on digester. The burning of biogas is done after the presure became constant. The optimal EM-4 activator addition for 20 litre of tofu wastewater substrate was 0.75%, with burning time is 14 minutes 56 seconds.
Search Region of Origin Honey Bee A. mellifera in Indonesia Region Using Mitochondrial DNA intergenic cox1/cox2 Mohamad Rusdi Hidayat
Biopropal Industri Vol 2, No 2 (2011)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36974/jbi.v2i2.731

Abstract

Apis mellifera is a favourite honey bee for the beekeepers throughout many countries. This species comprise of 24 subspecies. Based on phylogeography and morphometric evidences, these subspecies have been grouped into four lineage; namely the African (A), Western and Northern Europe (M), Southeastern Europe (C), and Near Eastern (O). Apis mellifera have been imported to Indonesia since 1972, and mostly from Australia. However, until recently there are no data about the A. mellifera subspecies and the origin. Therefore the objective of this research is to determine the lineage of A. mellifera in Indonesia based on mtDNA intergenic region between cox1/cox2 genes. In this region there are two DNA fragments, P and Q fragnant, that can be used to determine the A. mellifera lineage. The methodology used consist of samples collection, DNA isolation, DNA amplification, DNA restriction using DraI enzyme, DNA sequencing, and DNA alignment using Clustal X and MEGA spftwares. DNA fragment amplified by using E2 and H1 primer revealed a 863 bp. Digestion of the region with the DraI restriction enzyme revealed one haplotype, which consist of five DNA fragments. Based on DNA sequences and DNA alignment, A. mellifera in Indonesia was homologue with the C lineage. Its subspecies is A. m. ligustica that lived natively in Italy, they were imported to Indonesia from Australia
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