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Yoyon Suyono
Baristand Industri Pontianak
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Determination of Pseudomonas Bacteria Species and Analysis of Phylogenetic Tree using Bioinformatics Method Yoyon Suyono
Biopropal Industri Vol 1, No 2 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.66 KB) | DOI: 10.36974/jbi.v1i2.725

Abstract

Bioinformatics is the application of information technology in the field of biology by organizing and analyzing genetic data from a database of DNA, RNA and proteins into meaningful biological information. One role of bioinformatics is to identify the unknown bacteria and analysis of phylogenetic relationships based on genetic information is relatively easy, quick and cheap.The research aims to determine species and analyzed the phylogenetic tree bacteria of the genus Pseudomonas in Bioinformatics.  Research conducted through the stages of isolation, testing and continuing 16S rRNA bioinformatics studies include, for studies using the Basic Local Alignment Similarity Search Tool (BLAST) from NCBI, Multiple Sequence Alignment (MSA) using Clustal W from the EMBL-EBI and visualization in a phylogenic tree using the Tree View software. Isolation is obtained 2 (two) isolates the code 251 and 252. Testing 16S rRNA sequences of nucleotides in Fasta format. BLAST analysis in GenBank database of identity obtained respectively 99%, E-value 0.0 and the value of bits score above 50, Pseudomonas aeruginosa strains MZA-85 and Pseudomonas sp. J16. Multiple sequence alignment (MSA) and visualization of tree phylogenetic homologous isolates of code 251 and has a kinship with the species Pseudomonas aeruginosa Isolate 29 and isolate the code 252 with a species of Pseudomonas sp. J16 with a score of 99% and 100%. Isolates obtained by including the genus Pseudomonas species, Pseudomonas aeruginosa and Pseudomonas sp that has a nitrogen-fixing ability and resistance to the herbicide.
Preliminary Studies with Filler Organoclay Nanocomposite Making for Packaging Yoyon Suyono
Biopropal Industri Vol 3, No 2 (2012)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.732 KB) | DOI: 10.36974/jbi.v3i2.740

Abstract

Clay from West Kalimantan is still used as a raw material in the ceramic industry and it doesn’t have spesific processes for the manufacture of other products. The objective of this study is to determine the effect of organoclay filler on the physical properties of tensile strength clay nanocomposite food packaging applications. The step for clay nanocomposite were produced by purification, milling, organoclay, clay nanocomposite. Clay nanocomposite were characterized by analysis cation exchange capacities (CEC), X-ray diffraction (XRD), particle size analysis (PSA), scanning electron microscopy (SEM), tensile strength test and elongation at break. The purified with hydrochloric acid can increase the value of CEC and remove of the impurities. High energy milling (HEM) technique are with a time of 2 hours gained 225.3 nm sized nanoparticles as raw material organoclay. The addition of 1% organoclay filler increases the tensile strength of nanocomposite clay, according grade 2 and is suitable for second packaging or  not directly contact to food.
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