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Nadra Khairiah
Baristand Industri Banjarbaru
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Isolasi dan Uji Aktivitas Antimikroba Kapang Endofit dari Kayu Ulin (Eusideroxylon zwageri Teijsm & Binn.) (Isolation and Antimicrobial Activity of Endophytic Fungi from Ulin (Eusideroxylon zwageri Teijsm & Binn.)) Nadra Khairiah; Rinne Nintasari
Jurnal Riset Industri Hasil Hutan Vol 9, No 2 (2017)
Publisher : Kementerian Perindustrian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.642 KB) | DOI: 10.24111/jrihh.v9i2.3373

Abstract

Endophytic fungi are reported to be potential as antibacterial, antifungal, antioxidant, anticancer, and antivirus. One of the source of endophytic fungus is ulin (Eusideroxylon zwageri Teijsm & Binn.) which produced the same antimicrobial compound with ulin wood itself. The purpose of this research was to isolate endophytic caps from ulin wood branch, and tested the antimicrobial and antioxidant activity. The isolates were fermented on PDB media (Potato Dextrose Broth), and then tested the antimicrobial activity on Eschericiacoli, Pseudomonas aeruginosa, and Enterococcus aerogenes). In this research, there were two endofit isolates (white/PT and greenish black/HT). HT endophytic isolate exhibited strong antibacterial activity against the two pathogens (P. aeruginosa and E. coli) shown in 12 mm inhibition zone, while PT endophytic isolate exhibited the E. coli in 11.5 mm inhibition zone. The highest antioxidant activity found in HT endophytic shell was 47.47%. Keywords : antimicrobial, endophytic, Eusideroxylon zwageri, fungi, ulin
Aplikasi ekstrak sarang semut sebagai senyawa antimikroba dan antioksidan pada permen karet herbal [Application of ant nests (Myrmecodia sp.) extract as antimicrobial and antioxidant compounds in herbal gum] Nadra Khairiah; I Dewa Gede Putra Prabawa; Saibatul Hamdi; Nazarni Rahmi
Jurnal Riset Industri Hasil Hutan Vol 11, No 1 (2019)
Publisher : Kementerian Perindustrian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.941 KB) | DOI: 10.24111/jrihh.v11i1.4806

Abstract

Ant nests (Myrmecodia sp.) are one of the medicinal plants that have many benefits for health. Ant nests are widely used as antimoicrobial, antidiabetic, anticancer and antiimplantation because ant nests usually contains chemical compounds such as flavonoids, terpenoids, saponins and polyphenol tannins. Flavonoids can be used as antioxidants, antiangiogenic, and antimicrobials. Many products can be developed from the extract, such as tea, powder and ant nest capsules. Another product innovation that needs to be developed is gum. It is important to conduct research to determine the optimum formulation of ant nest extract as an additional ingredient on candy products (gum). This study aims to determine the antimicrobial and antioxidant activities of ant nests extract and maximize the use of ant nest extractas at additional raw material for high quality gum. The ant nests were extracted using 70% ethanol. The concentrated extract was tested for antimicrobial activity in four types of pathogenic bacteriaEscherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, Pseudomonas aeruginosa ATCC 9027, and Salmonella typhimurium ATCC 14028); antimicrobial positive control was tested using chloromphenicol and antioxidant activity positive control was tested with ascorbic acid (vitamin C). Dried ant nest extract was added to the gum mixture as much as 0.15% and 3% and then the quality was tested based on the parameters according to the quality standard of gum. The results of this study showed that ant nest extract had effective antibacterial activity in gram-positive and gram negative bacteria with an inhibitory zone of 10-13.5 mm. The high antioxidant activity of ant nests (85.90%) proven that ant nests had the potential as an natural antioxidants added in food. The addition of 0.15% and 0.3% extracts in chewing gum generally produced gum that met the quality standards of chewing gum, although the moisture content was slightly higher than the maximum limit of the standards.
Co-Authors I Dewa Gede Putra Prabawa Nazarni Rahmi Rinne Nintasari Saibatul Hamdi