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Tek An Lie
Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University
Agriculture, Biological Sciences & Forestry

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CHARACTERISATION OF SOYBEAN RHIZOBIAL STRAINS FROM JAVA AND SUMATRA Waluyo, Setiyo Hadi; Lie, Tek An; Mannetje, Leendert’t; de Vos, Willem M.
Indonesian Journal of Agricultural Science Vol 6, No 1 (2005): April 2005
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

To get insight in the structure of soybean rhizobial populationnative to Indonesian soils, a thorough survey of the occurrenceof the soybean rhizobia were conducted in several locations inJava and Sumatra. A total of 51 different isolates of rhizobialstrains were characterised phenotypically based on their symbioticproperties, and genetically using amplified ribosomal DNArestriction analysis (ARDRA). Based on their nodulation capacityon both soybean and the native legume mungbean, these rhizobialstrains could be divided into a group of 16 strains specific forsoybean only and another group of 35 promiscuous strains thatnodulated both leguminous plants. Based on ARDRA of PCRamplified16S rDNA and 16S-23S rDNA spacer fragments, therhizobial strains isolated from Java differed with those fromSumatra. Six Java isolates and only one Sumatra isolate wereclassified as Bradyrhizobium japonicum and these similar to thatof B. japonicum strain USDA 110. All these B. japonicum strainswere highly specific for soybean. One isolate from Java showeda rather unique position. The remaining strains from Java (20),which were symbiotically promiscuous strains, were clustered inanother group. This group and another group containing mostSumatra isolates were distinct from B. japonicum USDA 110 andtherefore it is tempting to speculate that these represent indigenoussoybean rhizobial bacteria. Application of agriculturalpractices, such as enhancement of rhizobial population, toincrease soybean production is still essential and noteworthy inSumatra.
EFFECT OF PHOSPHATE ON NODULE PRIMORDIA OF SOYBEAN (Glycine max Merrill) IN ACID SOILS IN RHIZOTRON EXPERIMENTS Waluyo, Setiyo Hadi; Lie, Tek An; Mannetje, Leendert ’t
Indonesian Journal of Agricultural Science Vol 5, No 2 (2004): October 2004
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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To clarify whether P had a direct or indirect effect on the nodulation  process of soybean grown in acid soils from Sitiung, West Sumatra, Indonesia, a series of rhizotron experiments, with special attention given to formation of nodule primordia, was conducted at Laboratory of  Microbiology, Wageningen University in 1998-2000. It was shown that Ca and P were essential nutrients for root growth, nodule formation, and growth of soybean in the acid soils (Oxisols). Ca increased root growth, number of nodule primordia, nodules, and growth of the soybean plant. This positive effect of Ca was increased considerably by the application of P. Ca and P have a synergistic effect on biological nitrogen fixation (BNF) of soybean in acid soils. Ca is importantfor the establishment of nodules, whilst P is essential for the development and function of the formed nodules. P increased number of nodule primordia, thus it also has an important role in the initiation of nodule formation. From this study, it can be concluded that Ca and P are the most limiting nutrients for BNF of soybean in the acid soils of Sitiung, West Sumatra, Indonesia.
Co-Authors Leendert ’t Mannetje Leendert’t Mannetje Setiyo Hadi Waluyo Willem M. de Vos