Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Makara Journal of Science
SAMUEL LIHAN
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan 94300, Sarawak, Malaysia

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Isolation and Identification of Airborne Bacteria Inside Swiftlet Houses in Sarawak, Malaysia Sien, Leong Sui; Chuan, Chia Hwa; Lihan, Samuel; Yee, Ling Teck
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Air consists of such microorganisms as bacteria, fungi, and viruses. Exposure to these airborne bacteria indoors may cause infectious and noninfectious adverse health effects. However, the sources and origins of bacteria are not fully understood. The aim of this study was to isolate and identify the bacteria present in the air inside swiftlet houses located in Kota Samarahan, Saratok, Betong, Maludam, Miri, Kuching, Semarang, Sepinang, Sarikei, and Sibu in Sarawak, Malaysia. A total of 100 bacterial isolates from 20 samples were collected from swiftlet houses. The bacteria present in the air were collected using Plate Count Agar. Two plates were exposed at the front and back inside swiftlet houses for 15 sec and then incubated at 37 ± 1 oC for 24 h. The 16S rRNA analysis method was used to identify the isolates from the samples. The air inside the swiftlet houses had a total mean airborne bacteria colony count of 2.02 ± 0.72 log10 cfu/m2 /sec; the highest was in Miri (3.08 ± 0.29 log10 cfu/m2 /sec), and the lowest was in Sibu (1.05 ± 0.85 log10 cfu/m2 /sec). Twenty-seven bacteria species were identified, and Lysinibacillus sp. B4 (16%) was most frequently isolated
Enzymatic Screening and Genotypic Characterization of Thermophilic Bacteria from the Hot Springs of Sarawak, Malaysia Toh, Seng Chiew; Lihan, Samuel; Leong, Sui Sien; Lahuri, Azizul Hakim; Woon, Wai Cheong; Ng, Wing Woh
Makara Journal of Science Vol. 27, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Owing to their eccentric thermostable ability, thermophiles are among the most utilized extremophiles in various industries, such as manufacturing, and clinical research. Researchers believe that many unknown thermophiles are yet to be discovered. This study aimed to genotypically characterize the diversity of thermophiles and screen them for the potential production of enzymes in the recreational hot springs located at Northwest Coast of Borneo. Water samples were collected at 45 °C–50 °C from Annah Rais and Panchor hot springs during the sampling period from January 2018 to January 2019. Three samples (water and sediment) were collected twice in a 3-week interval from each pool of the sampling sites. Each water sample was diluted up to 10−3 and plated on thick nutrient agar at 55 °C for 24 h. Customized nutrient agar plus Bacto-agar plates were used for the optimum growth analysis of the isolates at 40 °C–90 °C for 24 h. The thermophiles were isolated, characterized biochemically, and amplified molecularly using DNA fingerprinting and 16S rRNA gene sequencing. Lipase, protease, gelatinase, amylase, catalase, and nitrate reductase enzymatic production was examined. Twenty-one thermophilic isolates were successfully characterized into seven clusters of Amnoxybacillus spp. and Geobacillus spp. by studying their phylogenetic dendrograms. Isolates AR10 and AR15 could produce most of the tested enzymes. All the isolates showed negative results in gelatinase and lipase production. PC14 was the only isolate that did not produce any of the enzymatic reactions in this experiment. The results showed that most of the thermophiles isolated from the two Borneo hot springs can synthesize enzymes and have potential to be thermostable. In conclusion, the search for the thermophilic producers of novel enzymes in Borneo is successful; further research must focus on their applications.
Co-Authors CHIA HWA CHUAN Lahuri, Azizul Hakim LEONG SUI SIEN Leong, Sui Sien LING TECK YEE Ng, Wing Woh Toh, Seng Chiew Woon, Wai Cheong