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V.Y. Susilo
Center for Radioisotopes and Radiopharmaceuticals, National Nuclear Energy Agency Puspiptek Area , Serpong, 15314 Indonesia
Materials Science & Nanotechnology

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Synthesis of Sulochrin-125I and Its Binding Affinity as α-Glucosidase Inhibitor using Radioligand Binding Assay (RBA) Method W. Lestari; V.Y. Susilo; S. Setiyowati; Triningsih Triningsih; A. Ariyanto; P. Widayati; L.B.S. Kardono; A. Yanuar
Atom Indonesia Vol 40, No 1 (2014): April 2014
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.201 KB) | DOI: 10.17146/aij.2014.261

Abstract

Most of diabetes patients have type 2 diabetes mellitus or non insulin dependent diabetes mellitus. Treatment of type 2 diabetes mellitus can be conducted by inhibiting the α-glucosidase enzyme which converts carbohydrates into glucose. Sulochrin is one of the potential compounds which can inhibit the function of α-glucosidase enzyme. This study was carried out to obtain data of sulochrin binding with α-glucosidase enzyme as α-glucosidase inhibitor using Radioligand Binding Assay (RBA) method. The primary reagent required in RBA is the labeled radioactive ligand (radioligand). In this study, the radioligand was sulochrin-125I. Prior to the sulochrin-125I synthesis, sulochrin-I was synthesized. Sulochrin-I and sulochrin-125I were synthesized and their bindings were studied using RBA. The  molecular formula of the synthesized Sulochrin-I is C17H15O7I and its molecular weight is 457.9940. The sulochrin-125I was synthesized from sulochrin-I by isotope exchange method. From RBA, the dissociation constant (Kd) and the maximum binding (Bmax) were found as 26.316 nM and 9.302 nM, respectively. The low Kd indicates that sulochrin is capable of binding to α-glucosidase.Received: 08 November 2013; Revised: 28 February 2014; Accepted: 28 March 2014
Co-Authors A. Ariyanto A. Yanuar L.B.S. Kardono P. Widayati S. Setiyowati Triningsih Triningsih W. Lestari