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M. Syaifudin
Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12070, Indonesia
Materials Science & Nanotechnology

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In Vitro Infectivity Study of Cryopreserved Irradiated Intraerythrocytic Form of Plasmodium falciparum S. Nurhayati; T. Rahardjo; D Darlina; D. Tetriana; T. Kisnanto; M. Syaifudin; D. Ramadhani
Atom Indonesia Vol 43, No 3 (2017): December 2017
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.058 KB) | DOI: 10.17146/aij.2017.760

Abstract

In control human malaria infection studies using irradiated Plasmodium falciparum, the cell bank of irradiated P. falciparum infected erythrocytes is needed. The cell banking methods represent an obvious way to obtain suitable material for blood stage Plasmodium. In a cell bank development of irradiated Plasmodium infected erythrocytes, the ability to cryopreserve procedure of Plasmodium is important to recover the infectivity of irradiated Plasmodium. This study aims at evaluating the in vitro infectivity of cryopreserved irradiated intra-erythrocytic form  P. falciparum. A protein profile investigation using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of cryopreserved  P. falciparum also conducted in this study to know the cryopreserved effect on the protein of irradiated P. falciparum. Plasmodium falciparum of 3D7 strain in human erythrocytes was maintained in invitro continuous culture. When the percentage of parasites was 10-20%, the culture was harvested and irradiated with gamma rays at a dose of 175 Gy. Irradiated P. falciparum then was mixed with cryopreserved solution and stored in -80 °C for one hour before transferred into liquid nitrogen for 20, 40 and 60 days. After being stored the irradiated P. falciparum was thawed and cultured for 20 days. The percentage of parasitaemia was enumerated by examining Giemsa stained thin blood films prepared for 20 days after initiation of culture. Results showed that storage time significantly (p<0.05) influence the percentage of parasitaemia. The cooling procedure and cryopreservation media may affect this study results. It also showed that there was insignificant difference of  P. falciparum protein profile in all storage times. Overall it can be assumed that the irradiated P. falciparum still kept their infectivity after stored in liquid nitrogen for 60 days. Further study using different cooling procedure and different formula of cryopreservation media with a longer storage time should be conduct to validate this study results.
Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village - A High Background Radiation Area in Indonesia D. Ramadhani; S. Purnami; S. Nurhayati; M. Lubis; D. Tetriana; W. Mailana; K. D. Purwanti; E. Pudjadi; I. Kashiwakura; R. Okazaki; M. Syaifudin
Atom Indonesia Vol 45, No 1 (2019): April 2019
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/aij.2019.724

Abstract

People living in high background radiation area (HBRA) possibly developed the radioadaptive response (RAR) phenomena. Mamuju area in West Sulawesi Indonesia also known as the HBRA in Indonesia due to high natural uranium contents. It is possible that RAR was developed in Mamuju inhabitants. To prove this hypothesis, here in this study evaluation of the individual radiosensitivity in Takandeang Village, Mamuju inhabitants using G2 micronucleus (MN) assay was conducted. Association between blood groups and TP53 Arg72Pro polymorphism with individual radiosensitivity also evaluated in this study. Using G2 MN assay we assessed the individual radiosensitivity of Takandeang Village inhabitants and control samples. For each sample, three parameters were calculated. The spontaneous (baseline) MN number, MN number after 0.5 Gy in vitro irradiation and radiation induced MN were calculated to predict the individual radiosensitivity. The radiation induced MN was defined by subtracting the spontaneous MN number from the MN number after irradiation. The mean and SD of the number of micronucleus induced by radiation found in control group (CG) was set as the cut-off value to determine the individual radiosensitivity in all samples. The radiation induced MN higher than the mean CG + 1SD CS was scored as 1, indicating a milder radiosensitive phenotype, whereas a result higher than the mean CG + 2SD CG was scored as 2, and indicated a more severe radiosensitive phenotype. When the individual value was lower than the mean CG + 1SD CG, a score of 0 was attributed to the tested subject. Results showed that four individuals in Takandeang Village inhabitants were having a milder radiosensitive phenotype, whilst the other was categorized as normal radiosensitive. The similar finding also found in control samples. Our study failed to find a correlation between radiosensitivity and blood group also the TP53 Arg72Pro polymorphism. Overall our study revealed the possibility of RAR phenomena in Takandeang Village inhabitants. Further investigation using a different point of radiation dose value and larger sample number should be performed to validate this study results.        
Co-Authors D Darlina D. Ramadhani D. Ramadhani D. Tetriana E. Pudjadi I. Kashiwakura K. D. Purwanti M. Lubis R. Okazaki S. Nurhayati S. Purnami T. Kisnanto T. Rahardjo W. Mailana