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Sudjadi Sudjadi
Fakultas Farmasi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Kloning Gen cry dari Bacillus turingiensis var. kurstaki Nur Rahmi Ardiani; Sismindari Sismindari; Sudjadi Sudjadi
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 2 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9057.993 KB) | DOI: 10.22146/jpti.9918

Abstract

Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene.
Co-Authors Nur Rahmi Ardiani Sismindari Sismindari