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Suharto Suharto
Fakultas Pertanian Universitas Jember
Agriculture, Biological Sciences & Forestry

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Kajian Aspek Fisiologik Beauveria bassiana dan Virulensinya terhadap Helicoverpa armigera Suharto Suharto; Endang Budi Trisusilowati; Hari Purnomo
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 2 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9919

Abstract

Study on physiological aspects of six isolates of Beauveria bassiana and their virulence to Helicoverpa armigera was conducted in laboratory. Six isolates were obtained from different host and geographical locations. Stocks cultures was freeze dried and when required they were reconstituted and placed onto fresh SDA and incubated for seven days. The result of research indicated that color of all colony are white. Colony growth rate was affected by kind of the media. The highest colony growth was found from rice bug Jember isolate (JeLa). The hyphal growth rate per days was significantly different between isolates. The highest hyphal growth rate was found from coffee berry borer Jember isolate (JeHh). The number of spore per ml in SDA was relatively higher than PDA. The number of spore was significantly different between isolates both in SDA and PDA. The highest number of spore was found from JeLa and JeHh in SDA and PDA, respectively. The germination of spore 24 hours after inoculation was found from JeLa and significantly different than other isolates, although the rate of germination per hour was not significantly different. The number of spore germination was increased by temperature change from 27oC to 45oC. However, the increase of temperature up to 50oC, the number of spore germination become lower than 27oC. Spore germination was decreased by irradiation of UV light. Among six isolates, the highest virulence to H. armigera was found from JeLa isolate.
Patogenisitas Beberapa Isolat Beauveria Bassiana pada Plutella xylostella Suharto Suharto
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12207

Abstract

Plutella xylostella (L.) (Lepidoptera : Plutellidae) is key pest on cabbage. P. xylostella can be infected by Beauveria bassiana from other insects. The objective of this study was to determine the pathogenicity five isolates of B. bassiana on P. xylostella. Those isolates were BbUj1 (larvae of Lepidoptera), BbUj2 (rice brown plant hopper), BbUj3 (rice brown plant hopper), BbUj4 (rice seed bug), and BbUj5 (rice black bug). In the laboratory every isolate was inoculated 5 µL spore suspension at rate 107 spores/mL to third instar P. xylostella larva used micro liter syringe. Each treatment used 10 larvae and was replicated five times. The pathogenicity of B. bassiana was determined based on mortality and LT50 value. The potential isolate was tested to find LC50 subsequently tested on cabbage crop in the green house. The result of the study showed that BbUj1 from larvae of Lepidoptera was the potential isolate with LC50 of 9.49 x 10^5 spores/mL. LT50 was 2.67 days for the concentration 107 spores/mL. Green house application of B. bassiana at rate of 5 x 10^12 spores/ha caused 57 percent mortality of P. xylostella larva in seven days after treatment and LT50 was 5.67 days.
Co-Authors Endang Budi Trisusilowati Hari Purnomo