Y. M. S. Maryudani
Fakultas Pertanian Universitas Gadjah Mada

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Karakterisasi Parsial Streptomyces spp., Agens Pengendali Hayati Peyakit Lincat Tembakau Triwidodo Arwiyanto; Ari Astuti; Y. M. S. Maryudani
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11854

Abstract

Local isolates of Streptomyces spp. were proven could suppress "lincat disease" of tobacco in the field. Six isolates were chosen for partial characterization of their bacteriological properties as based for the next experiments purposes. The results indicated that the isolates produce miselium with spore chains, gram positive, aerob, catalase and oxidase positive. The isolates also hydrolize starch, gelatine and esculine; produce lecithinase enzyme, reduce nitrate to nitrite, do not produce melanine pigment, did not produce hydrogen sulfide. The isolates were sensitive against streptomycine and rifampicin; able to use several carbon and nitrogen sources tested. Capable to grow on several medium pH, from 4,3 to 8,0. The isolates were able to grow from 5° C to 45° C; able to grow on medium containing 4% to 7% NaCl and ion the medium containing 0,1% of phenol. Plant pathogenicity test result showed negative responses which indicated that the used isolates were non plant pathogenic. The ability in suppressing lincat pathogen (Ralstonia solanacearum and Meloidogyne incognita) in vitro was vary between isolates.
Pengaruh Perlakuan Uap Air Panas dengan Sistem Pemanasan Terbuka terhadap Kesehatan dan Viabilitas Benih Jagung Singgih Kurniawan; Ani Widiastuti; Y. M. S. Maryudani
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11884

Abstract

The existence of seed-borne fungi could degrade the seed quality in its viability and may cause disease after planting. The aim of this research was to know the hot vapor treatment effectiveness in controlling that fungal disease and its influence to the viability of corn seed. The treatment effectiveness was measured based on the reduction of the fungal growth and sporulation on Potato DextroseAgar (PDA), the fungal infection on seed, and not reduced the seed viability significantly after treatment. Hot vapor treatment was done in 50°C, 60°C, and 70°C temperature for 20 minutes and a control. The temperature treatments cover 5 minutes of antecedent treatment in the form of appliance warm-up until the target temperature obtained, 10 minutes in the treatment drum and 5 minutes for resting time while the faucet is being shut down but the exhaust fan remain to be opened. Result of the research show that in vitro test of hot water vapor on 60oC and 70oC killed Aspergillus flavus, A. niger, Fusarium sp., and Penicillium sp. isolates. Both of the temperatures reduced the corn seed viability significantly. Infection of A. flavus still dominant in corn seed after treated on the three temperatures.The hot vapor treatment with 50oC is good for seed treatment of 408.9 g corn seed although the seed had been stored for about 9 months.