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Daisy Prapto Sriyanti
Fakultas Pettanian UPN Veteran Yogyakarta
Agriculture, Biological Sciences & Forestry

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Pemanfaatan Antibodi Monoklonal dalam I-ELISA untuk Deteksi Penyebab Penyakit Busuk Pucuk Kelapa (Phytophthora palmivora) Susamto Somowiyarjo; Daisy Prapto Sriyanti; Mulyadi Mulyadi; Suryanti Suryanti; Y. M.S. Maryudani; Bambang Hadisutrisno
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 2 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12762

Abstract

Mice monoclonal antibodies (MCAs) against Phytophthora palmivora, Butl. (the pathogen of coconut bud rot) have been produced and tested. The selected hybridoma (Ab-PM.3) secreted monoclonal antibodies of IgM subclass and the titer in vitro of 10^2 - 1O^3, was employed to develop Indirect-Enzyme Linked lmmunosorbent Assay. This assay was able to differenciate coconut isolate of P. palmivora not only from cocoa and pepper isolate of the same species, but also from other fungus isolated from coconut bud rot diseased coconut (Fusarium sp., Colletotrichum sp., and Thielaviopsis sp.). This assay was able to differenciate P. pulmivora isolate from diseased coconut with those isolated from cocoa and pepper, and other fungus isolated from diseased coconut, thereby providing specific diagnostic tool for coconut bud rot which is needed for supporting the application of the concept of integrated pest mangement on coconut.
Co-Authors Bambang Hadisutrisno Mulyadi Mulyadi Suryanti Suryanti Susamto Somowiyarjo Y. M.S. Maryudani