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All Journal Samakia: Jurnal Ilmu Perikanan
Ismaningdyah Kurniawati
Program Studi Pengolahan Hasil Perikanan, Akademi Perikanan Ibrahimy, Situbondo.
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Other

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PENENTUAN RANGE DOSIS IMUNOSTIMULAN DAN LAMA WAKTUPERENDAMAN TERBAIK PADA EKSTRAK KASAR FENOLGracilaria sp. SEBELUM UJI TANTANG BAKTERI Aeromonas sp. DENGAN MENGGUNAKAN LC50 Ismaningdyah Kurniawati; Maftuch Maftuch; Anik Martinah Hariati
Samakia : Jurnal Ilmu Perikanan Vol 8 No 1 (2017): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.046 KB) | DOI: 10.5281/jsapi.v8i1.266

Abstract

Immunostimulatory dose given to the fish to be precise, should not be less and should not be excessive. This is because the immunostimulatory properties which can only work at the optimal dose.Therefore,themethod fordetermining the dosage and administration techniques immunostimulatory should be studied in depth.Research on the determination of the dose and the best maceration time on the crude extract phenol Gracilaria sp. using LC50 is intended to determine how best dose and how long it's best used in the immersion process to extract.rangedose used is 1 g/l; 1,5 g/l; 2 g/l; 3 g/l; 4 g/l and 5 g/l.The results of these observations indicate that doses ≥ 3 g/l has a 10-hour LC50.While the dose ≤ 3 g/l has a 14-hour LC50.However, further research is continued in the time range for prolonged submersion namely 10, 12 and 14 hours.The results showed the soaking time 12 hours showed LC50 15 hours.It concluded that the dose that can be used to test immunostimulant is 1, 1.5 and 3 g/l with a soaking time of less than 12 hours.
DETERMINATION OF THE BEST SOLVENT AND EXTRACT DURATION ON THE TECHNIQUE OF Gracilaria sp. MACERATION AS WELL AS ITS INFLUENCE ON MOISTURE CONTENT AND YIELD Ismaningdyah Kurniawati; Maftuch Maftuch; Anik Martinah Hariati
Samakia : Jurnal Ilmu Perikanan Vol 7 No 2 (2016): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.405 KB) | DOI: 10.5281/jsapi.v7i2.306

Abstract

Gracilaria sp. is one type of red algae that has many benefits. It is widely used as a cosmetic, gelatin and medicinal materials. . In the fisheries, especially in the field of aquaculture, utilization of algae has been quite widely used. One of them is the use of red algae as a madicine ingredient and immunostimulant material to minimze the use of antibiotics in fish. Research on determination of solvent extraction and extract duration done to know the best of solvent extraction and extract duration used as a reference to determine the time of the maceration when the Gracilaria sp. extraction process. The appropriate solvent expected to produce high yield (results of extraction). The type of solvent used is ethanol 80%, ethanol 96%, acetone and aquades. The range of time that is used for the maceration is 24, 48 and 72 hours. The results showed that the best type of solvent is ethanol 96% with long soaking for maceration during 48 hours. The resulting yield is 0.9 with a moisture content of 10%.
Co-Authors Hariati, Anik Martinah Maftuch Maftuch