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All Journal Journal of Tropical Soils BERITA BIOLOGI
Sri Purwaningsih
Bidang Mikrobiologi, Puslit Biologi-LIPI, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Population of Rhizobium bacteria in the soil at several plant from Buton island, Muna regency, Southeast Sulawesi Sri Purwaningsih
JOURNAL OF TROPICAL SOILS Vol 14, No 1: January 2009
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2009.v14i1.65-70

Abstract

The research was conducted in order to know population of Rhizobium bacteria in the soil at several plant from Buton island, Muna regency, Southeast Sulawesi. The purpose of the study was to get the population data and pure cultures of Rhizobium bacteria.Rhizobium bacteria were isolated from 13 sample from rhizosphere of fruit plant, 14 sample soil from rhizosphere of yield plant, 5 sample soil from rhizosphere of horticulture plant, and 8 sample soil from forest plant. Isolation was done in standard medium Yeast Extract Mannitol Agar (YEMA), the inculation at (27-280C), and the population with plate count methods. The growth characteristic of strain was observed by using YEMA medium mixed respectively with Brom Thymol Blue and Congo Red as indicators.The population of Rhizobium bacteria was in the range of 7-115 X 105 CFU/g soil, and the highest population was found from the sample soil from rhizosphere with Ipomea batatas plant. Fourty nine gave of pure culture, thirty five isolates can be grouped as fast growing, while fourteen can be grouped as slow growing.
PENGARUH INOKULASI RHIZOBIUM TERHADAP PERTUMBUHAN TANAMAN KEDELAI (Glycine max L) VARIETAS WILIS DI RUMAH KACA Sri Purwaningsih
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1867

Abstract

Effect of Rhizobium inoculation on the growth of Glycine max L. Wilis variety was endicated based on green house experiment.The aim of the experiment was to asses the potency of the Rhizobium strain to inccrease the growth of Glycine max L.Strains of : 1 WG, 1 WS, 1 P, 16 GH, 1 LS, 1 BK, 1 KT, 1 BR, and 11 WY were used in this research. Controls treatment were uninoculated with Rhizobium strain -1 and without urea fertilizer (K1) and uninoculated and with urea fertilizer equal 100 kg ha (K2). The research design was Completely Randomized Design with three replications for each treatment.Experiment plants were harvested after 50 days.Parameters of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules, and “Symbiotic Capacity”.Results of this studies showed all of the Rhizobium inoculants able to form nodule. Strain 1 KT (isolate of soil from Kalampangan, Palangkaraya,Central Kalimantan) has given the best results on the growth of Glycine max L.
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