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All Journal Journal of Tropical Soils BERITA BIOLOGI
Sri Purwaningsih
Bidang Mikrobiologi, Puslit Biologi-LIPI, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Journal : Journal of Tropical Soils

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Population of Rhizobium bacteria in the soil at several plant from Buton island, Muna regency, Southeast Sulawesi Sri Purwaningsih
JOURNAL OF TROPICAL SOILS Vol 14, No 1: January 2009
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2009.v14i1.65-70

Abstract

The research was conducted in order to know population of Rhizobium bacteria in the soil at several plant from Buton island, Muna regency, Southeast Sulawesi. The purpose of the study was to get the population data and pure cultures of Rhizobium bacteria.Rhizobium bacteria were isolated from 13 sample from rhizosphere of fruit plant, 14 sample soil from rhizosphere of yield plant, 5 sample soil from rhizosphere of horticulture plant, and 8 sample soil from forest plant. Isolation was done in standard medium Yeast Extract Mannitol Agar (YEMA), the inculation at (27-280C), and the population with plate count methods. The growth characteristic of strain was observed by using YEMA medium mixed respectively with Brom Thymol Blue and Congo Red as indicators.The population of Rhizobium bacteria was in the range of 7-115 X 105 CFU/g soil, and the highest population was found from the sample soil from rhizosphere with Ipomea batatas plant. Fourty nine gave of pure culture, thirty five isolates can be grouped as fast growing, while fourteen can be grouped as slow growing.
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