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All Journal Jurnal AgroBiogen Nusantara Bioscience
Dwinita Wikan Utami
Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development. Jl. Tentara Pelajar No. 3A, Bogor 16111, West Java, Indonesia.
Agriculture, Biological Sciences & Forestry Other

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Molecular Breeding for Developing A New Upland Rice Variety ‘Bio Patenggang Agritan’ Dwinita Wikan Utami; Maulidia Rahmawati; Siti Yuriyah; Ahmad Dadang; Siti Nurani; Nurul Jadid
Jurnal AgroBiogen Vol 15, No 1 (2019): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n1.2019.p1-10

Abstract

Situ Patenggang is an elite upland rice variety well-accepted by farmers, but recently this variety started to be attacked by blast disease (Pyricularia grisea) that causes yield loss up to 50%. To overcome this problem, Bio Patenggang variety has been developed through molecular breeding using blast-resistant monogenic lines as donor parents. The aim of this research was to present molecular breeding approach of Bio Patenggang development, including steps from the phenotypic selection on candidate lines to the genotypic analysis assisted by molecular markers. The phenotypic performances were assessed using Distinctness, Uniformity, and Stability (DUS) testing by following the standard method stated in the rice test guidelines. The genotypic analyses were performed using foreground and background markers. Four BC3F7 lines were selected as the candidates of the Situ Patenggang-derived variety. Based on ANOVA and Principal Component Analysis (PCA), phenotypic performances of the four lines demonstrated no significant differences with that of Situ Patenggang. The selected lines have also passed the foreground analysis confirming that the lines contained Pita, Pii, Pik-p, and Pia last resistance genes. Background analysis showed that the selected lines demonstrated agronomic perfomances very similar to that of Situ Patenggang. Association analyses showed that 14 markers were associated with the target traits and 10 out of the 14 markers were identified as co-segregation markers. The four selected lines, therefore, were proposed to be released as Situ Patenggang-derived variety. One of the lines (Sta-8-S15-TB16) has been approved to be released as a new variety, namely ‘Bio Patenggang Agritan’.
Evaluation of neck blast resistance and agronomical performances on double haploid rice population in greenhouse and endemic field GUT WINDARSIH; DWINITA WIKAN UTAMI
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090406

Abstract

Windarsih G, Utami DW. 2017. Evaluation of neck blast resistance and agronomical performances on double haploid rice population in greenhouse and endemic field. Nusantara Bioscience 9: 371-377. Blast disease caused by fungal Pyricularia grisea Sacc. is one of the most destructive diseases of rice in the world. The development of blast-resistant rice varieties will be essential to control this disease. This research aimed (i) to compare the resistance response to neck-blast among DH lines from double cross IR54/Parekaligolara//Bio110/Markuti and the differential varieties against three selected Indonesian blast races in greenhouse, (ii) to identify the gene(s) that caused the resistance to neck-blast based on the association between the resistance response and the genotype evaluation using molecular markers linked to Pi1, Pi33, Pib, Pir4 and Pir7 genes, and (iii) to evaluate the resistance response to leaf and neck blast on DH lines in endemic field (Sukabumi) and the agronomical performance of selected DH lines in optimum field in Ciasem of Subang, West Java, Indonesia during December 2013 to March 2014. Eleven double haploid lines from double-crossing IR54/Parekaligolara//Bio110/Markuti, the differential varieties as resistant control and the US2 variety for susceptible control were observed for neck-blast resistance response to three blast races in greenhouse and endemic field (Sukabumi), while the agronomical performances were observed in field of Ciasem-Subang. The results based on the genotyping evaluation, leaf and neck blast resistance, either in greenhouse and endemic location, and the agronomical performance in field showed that 5 selected double haploid lines had leaf and neck blast resistance and good performance on field trial. Thus they are promising for use either for further testing forwarding into releasing variety or used as donor for further blast resistant breeding activities.
Co-Authors Ahmad Dadang GUT WINDARSIH Maulidia Rahmawati Nurul Jadid Siti Nurani Siti Yuriyah