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Agung Prasetyo
Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
Chemistry

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Isolasi Dan Karakterisasi Lipase Dari Rebung Bambu Betung Dendrocalamusasper Agung Prasetyo; Winni Astuti; Chairul Saleh
JURNAL KIMIA MULAWARMAN Vol 15 No 1 (2017)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v15i1.585

Abstract

This research was conducted to get the extract of rough lipase produced by bamboo shoots (Dendrocalamus asper). Lipase isolation from bamboo shoots was done by blending bamboo shoots to produce the crude extract of the enzyme. Lipase crude extracts that occurred at centrifugation at 12000 rpm for 30 minutes at 4°C. The crude extracts obtained were then tested using quantitative pH 7, temperature 30ºC and substrate 1% (v/v). Based on research there is activity of crude extract of lipase equal to 2,24 U/ml.
Co-Authors Chairul Saleh Winni Astuti