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Hendric Surya Putra
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Chemistry

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AKTIVITAS AMILASE, PROTEASE dan LIPASE DARI MADU LEBAH Trigona sp, Apis mellifera dan Apis dorsata Hendric Surya Putra; Winni Astuti; Rudi Kartika
JURNAL KIMIA MULAWARMAN Vol 16 No 1 (2018)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v16i1.433

Abstract

Activities of amylase, protease and lipase from honey Trigona sp, Apis mellifera and Apis dorsata, determination protein concentration and the activity protease done with Bradford method, the determination of the glucose standard and activity amylase done with 3,5-dinitro salicylic acid (DNS) method and activity lipase done with acid-base titration with coconut oil substrate. The honey from Trigona sp has value of the activity amylase and lipase respectively by 0,0136 U / mg and 0,359 U/mg, whereas honey Apis mellifera has activity of protease, amylase and lipase of each 1,22 x 10-6 U/mg; 0,944 U/mg and 0,304 U/mg and then honey Apis dorsata has amylase and lipase activity of each of 0,0645 U/mg and 0,287 U/mg.
Co-Authors Rudi Kartika, Rudi Winni Astuti