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Mahardika Agus Wijayanti Mahardika Agus Wijayanti
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Secretion of tumor necrosis factor and reactive oxygen intermediates from soluble antigens of Plasmodium falciparum stimulated-peritoneal mouse macro-phages Mahardika Agus Wijayanti, Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 01 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.113 KB)

Abstract

Background: Malarial infection is stil one of major health problems in the world. In Indonesia, malarial infection is especially caused by Plasmodium falciparum and Plasmodiun vivax. Host immune responses to malarial infection are complex mechanisms, including the humoral immunity by antibody and cellular immunity by T cells and activated effector cells. Macrophages as an effector cells kill malarial parasite by oxidative and non-oxidative mechanism. Tumor necrosis factor (TNF) and reactive oxygen intermediates (ROI) are mediators produced by macrophages which represent as non-oxidative and oxidative killing respectively.Objectives: Understanding the secretion ability of tumor necrosis factor and reactive oxygen intermediates from soluble antigens of P. falciparum stimulated-peritoneal mouse macrophages.Method: In this. study, soluble antigens of P. fa/p/parum stimulated-peritoneal mouse macrophages were tested to produce TNF and ROI in vitro. Secretion of TNF was measured by MTT assay dan ROI by NBT reduction assay. Swiss mice were divided into two groups of 15 mice each. One group was stimulated by soluble antigens as experimental group and the other as control group.Result: Secretion of TNF and ROI by soluble antigens of P. falciparum stimulated-peritoneal mouse macrophages were significantly higher (p<0,01) than control group.Conclusion: Soluble antigens of P. falciparum could activate mouse peritoneal cells in vivo. Therefore, mouse macrophages provide a convenient system for investigating the human malarial soluble antigens.Key. words : Soluble antigens of Plasmodium fa/ciparum - Cellular Immunity - Macrophages – Tumor - Necrosis Factor - Reactive Oxygen Intermediate
The effect of extract andropogon zizanioides urban roots as a repelent to Aedes Aegypti mosquito Mahardika Agus Wijayanti, Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 03 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.697 KB)

Abstract

Dengue haemorrhagic fever (DHF) is one of the viral infections transmitted by mosquitoes that still remains a health problem in Indonesia. Attempts to overcome this disease through experimental studies, in order to get rapid and right diagnosis, specific treatment and vaccine development have not been satisfactory. The main vector of DHF are Aedes aegypti and Aedes albopictus mosquito. Self protection againts mosquito bites could be done by using repelent. Lorosetu (Andropogon zizanioides urban) is a plant belonging to one family of fragrant grass, usually used as a soap fragrance or supplementary medication. Its root is commonly used to chase insect In the wardrobe. The aim of this study was to Investigate the effect of A. zizanioides Urban extract as a repelent to A. aegypti mosquito in the laboratory. In this study, time series observation using various concentration of extract A. zizanioides Urban as a repelent to A. aegypti was done. The result analized by Spit-plot and T-test showed that 25% extract of A. zizanioides Urban roots has the effect as a repelent within one hour, while the 50% and 100% concentration have the effect within two hours.Key words: Dengue Haemorrhagic Fever - Aedes aegypti - root extract - Andropogon zizanioides Urban root - repellent
Phagocytic activity of immunized-mouse peritoneal macrophages during Plasmodium berghei infection Mahardika Agus Wijayanti, Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 04 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.567 KB)

Abstract

Background: Macrophage represents one of the cellular component of the immune system which plays an important role during malarial infection. Both the number and functional activities including phagocyte activity of these cells increase during the infection.Objectives: This study was carried out to investigate the phagocyte activity of peritoneal macrophages from immunized and non-immunized mice during P. berghei infection.Methods: Swiss mice were divided into two groups, one experimental group was immunized by crude vaccine P. berghei, one control group was not immunized. Phagocyte activity was measured by the ability of mouse peritoneal macrophages to phagocytes latex particles in vitro.Results: In non-immunized mice the percentage of macrophages which were phagocyte latex particles was increased during early infection, reached a peak of about 9 times of the normal level then declined until the mice died. In the immunized mice this activity was increased to reach a peak of about 11 times of the normal level and remained high until recovery.Conclusion: Phagocyte activity of immunized-mice peritoneal macrophages was significantly higher than those of non immunized. The increase of the phagocyte activity seemed to be correlated with the ability of mice to overcome the infection.Key words : Immunization - P. berghei - Effector cells - Macrophages - Phagocytosis
The effect of immunization of mice with blood stage parasite against Plasmodium berghei infection Mahardika Agus Wijayanti, Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 02 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.233 KB)

Abstract

Immunity against malarial infection is a very complex molecular and cellular interaction and represents a combination of both humoral and cell mediated mechanism. However, which mechanism contributes to the protection effect is still not clear. Immunization against Plasmodium berghei infection represents a suitable malarial model to study the host immune responses against malarial infection. The present study was aimed at evaluating the effect of immunization of Swiss mice challenged with P. berghei on the blast transformation, prepatent period, parasitemia and mortality of the host. The result showed that P. berghei infection in .Swiss mice was acute and fatal. All non-immunized mice died following challenge with 1 x 108 parasite on day 8 -10 post infection. Blast transformation of splenic lymphocyte was higher in immunized mice than in non-immunized mice. Immunization with P. berghei and adjuvant in Swiss mice could evoke partial immunity to homolog parasite. Longer prepatent period, reduced parasitemia and decreased mortality were observed in this group. Eighty percent of immunized mice survived from P. berghei infection.Key Words : malarial immunity - immunization - blast transformation - prepatent period - parasitemia -mortality
Phagocytic activity of immunized-mouse peritoneal macrophages during Plasmodium berghei infection Mahardika Agus Wijayanti Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 04 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.567 KB)

Abstract

Background: Macrophage represents one of the cellular component of the immune system which plays an important role during malarial infection. Both the number and functional activities including phagocyte activity of these cells increase during the infection.Objectives: This study was carried out to investigate the phagocyte activity of peritoneal macrophages from immunized and non-immunized mice during P. berghei infection.Methods: Swiss mice were divided into two groups, one experimental group was immunized by crude vaccine P. berghei, one control group was not immunized. Phagocyte activity was measured by the ability of mouse peritoneal macrophages to phagocytes latex particles in vitro.Results: In non-immunized mice the percentage of macrophages which were phagocyte latex particles was increased during early infection, reached a peak of about 9 times of the normal level then declined until the mice died. In the immunized mice this activity was increased to reach a peak of about 11 times of the normal level and remained high until recovery.Conclusion: Phagocyte activity of immunized-mice peritoneal macrophages was significantly higher than those of non immunized. The increase of the phagocyte activity seemed to be correlated with the ability of mice to overcome the infection.Key words : Immunization - P. berghei - Effector cells - Macrophages - Phagocytosis
Secretion of tumor necrosis factor and reactive oxygen intermediates from soluble antigens of Plasmodium falciparum stimulated-peritoneal mouse macro-phages Mahardika Agus Wijayanti Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 01 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.113 KB)

Abstract

Background: Malarial infection is stil one of major health problems in the world. In Indonesia, malarial infection is especially caused by Plasmodium falciparum and Plasmodiun vivax. Host immune responses to malarial infection are complex mechanisms, including the humoral immunity by antibody and cellular immunity by T cells and activated effector cells. Macrophages as an effector cells kill malarial parasite by oxidative and non-oxidative mechanism. Tumor necrosis factor (TNF) and reactive oxygen intermediates (ROI) are mediators produced by macrophages which represent as non-oxidative and oxidative killing respectively.Objectives: Understanding the secretion ability of tumor necrosis factor and reactive oxygen intermediates from soluble antigens of P. falciparum stimulated-peritoneal mouse macrophages.Method: In this. study, soluble antigens of P. fa/p/parum stimulated-peritoneal mouse macrophages were tested to produce TNF and ROI in vitro. Secretion of TNF was measured by MTT assay dan ROI by NBT reduction assay. Swiss mice were divided into two groups of 15 mice each. One group was stimulated by soluble antigens as experimental group and the other as control group.Result: Secretion of TNF and ROI by soluble antigens of P. falciparum stimulated-peritoneal mouse macrophages were significantly higher (p<0,01) than control group.Conclusion: Soluble antigens of P. falciparum could activate mouse peritoneal cells in vivo. Therefore, mouse macrophages provide a convenient system for investigating the human malarial soluble antigens.Key. words : Soluble antigens of Plasmodium fa/ciparum - Cellular Immunity - Macrophages – Tumor - Necrosis Factor - Reactive Oxygen Intermediate
The effect of extract andropogon zizanioides urban roots as a repelent to Aedes Aegypti mosquito Mahardika Agus Wijayanti Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 03 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.697 KB)

Abstract

Dengue haemorrhagic fever (DHF) is one of the viral infections transmitted by mosquitoes that still remains a health problem in Indonesia. Attempts to overcome this disease through experimental studies, in order to get rapid and right diagnosis, specific treatment and vaccine development have not been satisfactory. The main vector of DHF are Aedes aegypti and Aedes albopictus mosquito. Self protection againts mosquito bites could be done by using repelent. Lorosetu (Andropogon zizanioides urban) is a plant belonging to one family of fragrant grass, usually used as a soap fragrance or supplementary medication. Its root is commonly used to chase insect In the wardrobe. The aim of this study was to Investigate the effect of A. zizanioides Urban extract as a repelent to A. aegypti mosquito in the laboratory. In this study, time series observation using various concentration of extract A. zizanioides Urban as a repelent to A. aegypti was done. The result analized by Spit-plot and T-test showed that 25% extract of A. zizanioides Urban roots has the effect as a repelent within one hour, while the 50% and 100% concentration have the effect within two hours.Key words: Dengue Haemorrhagic Fever - Aedes aegypti - root extract - Andropogon zizanioides Urban root - repellent
The effect of immunization of mice with blood stage parasite against Plasmodium berghei infection Mahardika Agus Wijayanti Mahardika Agus Wijayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 02 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.233 KB)

Abstract

Immunity against malarial infection is a very complex molecular and cellular interaction and represents a combination of both humoral and cell mediated mechanism. However, which mechanism contributes to the protection effect is still not clear. Immunization against Plasmodium berghei infection represents a suitable malarial model to study the host immune responses against malarial infection. The present study was aimed at evaluating the effect of immunization of Swiss mice challenged with P. berghei on the blast transformation, prepatent period, parasitemia and mortality of the host. The result showed that P. berghei infection in .Swiss mice was acute and fatal. All non-immunized mice died following challenge with 1 x 108 parasite on day 8 -10 post infection. Blast transformation of splenic lymphocyte was higher in immunized mice than in non-immunized mice. Immunization with P. berghei and adjuvant in Swiss mice could evoke partial immunity to homolog parasite. Longer prepatent period, reduced parasitemia and decreased mortality were observed in this group. Eighty percent of immunized mice survived from P. berghei infection.Key Words : malarial immunity - immunization - blast transformation - prepatent period - parasitemia -mortality
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