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All Journal UNEJ e-Proceeding Biosaintifika: Journal of Biology & Biology Education
Hendro Pramono
Faculty of Biology, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Education

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HIGHLY SPESIFIC BACILLUS CEREUS-PHAGES ISOLATED FROM HOSPITAL WASTEWATER IN BANYUMAS REGENCY Anwar Rovik; Saefuddin ‘Aziz; Hendro Pramono
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Bacillus cereus is pathogenic bacteria that frequently contaminate food by producing entero and emetic toxins. B. cereus had shown resistance to various antibiotics. An alternative to control B. cereus is the use of bacteriophages. This study aims to determine whether bacteriophages isolated from hospital wastewater in Banyumas Regency is highly specific to B. cereus. The research was carried out descriptively through isolation, purification, enumeration, specificity, effectivity, and host-lysing rate test. B. cereus phages were isolated from hospital wastewater in Banyumas Regency with various titer, i.e. 108-109 PFU.ml-1. The PKRW-1, PKCL-1, PKSR-2, RSBMS-2, RSBMT-1, RSAJP-1, RSAJP-2, RSAJK-2, RSAJE-1, and RSAJE-3 phages have a great specificity and effectivity of infection to B. cereus. Total of 7 isolated phages have a fast host-lysing rate by the 2nd hour of incubation.
Potential of Chemolitotrophic Bacteria From Gold Mining Area in Sulfur Oxidation Process Nur Lu'lu Fitriyani; Agus Irianto; Hendro Pramono
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.12544

Abstract

Gold in nature is covered by rocks which contain sulfide minerals such as pyrite, chalcopyrite, arsenopyrite, and others sulfide minerals. Chemolithotrophic bacteria have the ability to oxidize the sulfur compounds and can be used in the process of releasing gold from carrier rocks which contain sulfide minerals. This research aimed to explore and identify the chemolithotrophic bacteria from gold mining areas as well as determine their potential for sulfur oxidation. The methods used in this study were exploring the potential of bacteria in sulfur oxidation and describing the variety of bacteria that were isolated from gold mining areas by 16s rRNA identification. The results showed that there were six isolates from isolation with Starkey solid medium, i.e. Bl-1, B1-2, B1-3, B1-4, B1-5 and B1-6 that were similar to Paenibacillus sp., Enterobacter ludwigiis train E8-13, uncultured Burkholderia sp., Uncultured bacterium clone N4.5, Bacillus subtilis strain CICC 10023, and Bacterium enrichment culture clone 02 respectively. The B1-3 isolate showed the highest increase of sulfate compound in the medium (8.04 % at 649.55 ppm). This indigenous bacteria will be able to be used to release gold from rock which contains sulfide minerals and reduce the use of hazardous chemicals commonly used in gold mining.
Co-Authors Agus Irianto Anwar Rovik Nur Lu’lu Fitriyani Saefuddin Aziz