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All Journal Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) UNEJ e-Proceeding
Lisa Soegianto
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Education Medicine & Pharmacology

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ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL OF ETHANOLIC EXTRACT FROM BINTARO FLOWER (Cerbera odollam) AGAINST Staphylococcus aureus ATCC 6538 Dwi Rahma Suci Lestari; Lisa Soegianto; Liliek S. Hermanu
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Staphylococcus aureus are examples of Grampositivebacteria that can cause infection.Treatment of infection with antibiotic synthesiscan bring its own problems, namely the resistanceof bacteria to antibiotics. Resistant bacteria is abacterial pathogen that is capable of formingbiofilms on living beings. Traditional medicine canbe another alternative in the treatment ofinfections. Natural compounds that have thepotential as an antibacterial commonly containsteroids, tannins, polyphenols, flavonoids1),alkaloids, saponins2).Some studies related to the use as an antibacterialBintaro explained that the mangrove plant thatbelongs to the family Apocynaceae is grown widelyin the southern coast of East Asia and the IndianOcean has antibacterial activity3).This study was conducted to determine theantibacterial activity and antibiofilm of ethanolicextract from Cerbera odollam flower againstStaphylococcus aureus.
EFFECTIVENESS OF BINTARO (Cerberra odollam Gaertn.) LEAF ETHANOLIC EXTRACT AGAINST Staphylococcus aureus IN-VITRO BIOFILM FORMATION Filania S. Kanja; Lisa Soegianto; Sumi Wijaya
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Biofilm is a structural form of a group ofmicroorganisms protected by matrik extracellularcalled Extracellular Polymeric Substance (EPS),where EPS is a product produced by thesemicroorganisms itself and as a shelter from thebad influence in environment. The structure ofthese matrix is strings which cross each otherwhich can be adhesive for biofilm4).Biofilm from Staphylococcus aureus seen inchronicle infection cases as ulcer on diabeticsfoot, venous statis ulcer and pressure sores.Patients with infection wound with chronicleulcer in vein feet found positive culture ofStaphylococcus aureus as much as 88-93,3% froma number of infection found.Figure 1. Cerberra odollam Gaertn.Bintaro can be used as an analgesic,anticonvulsant, kardiotonik and antihypertensive2).The aim of this study is to test the effectivenessof the ethanol extracts of bintaro leaves (Cerberraodollam Gaertn.) towards the Staphylococcusaureus formation by in vitro.MATERIALSBintaro leaves (Cerberra odollam Gaertn.),Staphylococcus aureus culture ATCC 6538, petridish, ose wire , micro pipet, autoclave (AllAmerican Model No 25x, USA), incubator(Memmert and Binder, Germany), Laminar AirFlow (LAF) (Type V-130, Indonesia), microplate,microplate reader (Thermofisher Scientific,America), vacuum rotary evaporator (BÜCHI,Germany), weight analytic (Sartorius TE 214 S,Germany), oven (Memmert, Germany).
Co-Authors Dwi Rahma Suci Lestari Farida L Darsono Filania S. Kanja Hamalatul Qur’ani Romelli Liliek S. Hermanu Sumi WIjaya, Sumi