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All Journal Jurnal Kedokteran Diponegoro Journal of Biomedicine and Translational Research
Puspita Kusuma Dewi
Department Of Medical Biology And Biochemistry, Faculty Of Medicine, Diponegoro University
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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THE EFFECT OF COFFEE ON THE SPERMATOZOA MOTILITY OF MALE WISTAR RATS (Rattus norvegicus) EXPOSED TO ELECTRIC CIGARETTE SMOKE Erika Shinta Avianty; Puspita Kusuma Dewi; Amallia Nuggetsiana Setyawati; Arufiadi Anityo Mochtar
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 3 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i3.29987

Abstract

Background: Man infertility was caused by decrease of spermatozoa quality. E-cigarette smoke which contained chemicals as nicotine could produce free radicals which decreased spermatozoa motility. Coffee was contained of caffeine and chlorogenic acid functioned as antioxidant and increase spermatozoa motility. Aim: To prove the coffee giving effect to spermatozoa motility of male wistar rats that have been exposed to e-cigarette smoke. Methods: The sample were 28 male wistar rats (Rattus norvegicus), no anatomical abnormalities, 200-300 grams, and 8-9 weeks. The sample was randomly divided into 4 groups: no treatment, the rats which were exposed to e-cigarette smoke, the rats which were given 3ml robusta coffee solution contained of 180mg coffee powder and exposed to e-cigarette smoke, the rats which were given 3ml robusta coffee solution contained of 360mg coffee powder and exposed to e-cigarette smoke. The coffees solution was given through a gastric swab. The e-cigarette liquid doze was 3,5ml with 18mg/ml nicotine for each group. After four weeks, the rat’s sperm sample was taken, preparation was made, and spermatozoa motility was examined through microscope. The statistical test exerted Saphiro-Wilks test and non-parametrical test from Kruskal-Wallis. Results: The mean of spermatozoa motility percentage in progressive, non-progressive, and negative control group immotility = (53,81±6,507), (30,00±5,444), (16,66±3,333); positive control = (0,48±1,259), (17,14±18,604), (82,38±19,787); first treatment = (10,00±4,304), (32,85±14,065), (57,14±17,259); second treatment = (11,42±1,779), (26,19±8,033), (62,38±8,760). Kruskal-Wallis test showed a significant difference in progressive motility and immotility (p=0,000). Conclusion: The coffee solution could give an effect on the spermatozoa motility of male wistar rats exposed to electric cigarette smoke.                                Keywords: Infertility, e-cigarette, nicotine, coffee, motility
The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models Puspita Kusuma Dewi; Maharani Cahyono; Riski Prihatningtias; Liana Ekowati; Arief Wildan
Journal of Biomedicine and Translational Research Vol 6, No 3 (2020): December2020
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v6i3.9414

Abstract

Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models.Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit.Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001).Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.
Co-Authors Amallia Nuggetsiana Setyawati Arief Wildan Arufiadi Anityo Mochtar Erika Shinta Avianty Liana Ekowati Maharani Cahyono Riski Prihatningtias