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H.P.S. Makkar
Institute for Animal Production in the Tropics and Subtropics (480b), University of Hohenheim, Fruwirthstrasse 12, 70593 Stuttgart
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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THE USE OF PRINCIPAL COMPONENT ANALYSIS IN IDENTIFYING AND INTEGRATING VARIABLES RELATED TO FORAGE QUALITY AND METHANE PRODUCTION Jayanegara, A.; Makkar, H.P.S.; Becker, K.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 4 (2009): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.4.241-247

Abstract

This research was aimed to explore the use of multivariate statistics i.e. principal componentanalysis (PCA) in identifying and integrating variables related to forage quality and ruminal methaneproduction, and in classifying forage species into both characteristics. Seventeen plants were used as adatabase for the above mentioned purposes. Plant samples were determined for their chemicalcomposition, cumulative gas production (represents the nutrient degradation) and methane productionafter 24 hours of fermentation period using the Hohenheim gas test. The results showed that the PCAcould clearly identify factors related to forage quality and methane production and separated them intodifferent principal components (PC). The obtained PC1 was related to methane production andsubstantially influenced positively by crude protein, NDF, ADF (positive), total phenols, total tannins,condensed tannins and tannin activity (negative). On the other hand, the obtained PC2 was related tocumulative gas production (forage quality) and substantially influenced by crude protein (positive),NDF, ADF and condensed tannins (negative). Classification and screening of forages that have highquality and low methane production are possible using the PCA technique. Rhenum undulatum,Peltiphyllum peltatum and Rhus typhina were found to have such desired characteristics.
EVALUATION OF LACTICIN ADDITION ON GAS PRODUCTION KINETICS AND RUMEN METHANOGENESIS in Vitro Jayanegara, A.; Makkar, H.P.S.; Becker, K.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.30-36

Abstract

The present study was aimed to investigate the effect of a novel bacteriocin, i.e. lacticin 3147, ongas production kinetics and methane emission under in vitro rumen environment in two consecutiveexperiments. In experiment 1, either no or 10 µM of lacticin was added to hay or hay:concentrate (1:1,w/w) substrate. In experiment 2, the levels of lacticin additions were extended to 0, 10, 25 and 50 µM.Samples were incubated in three replicates in both experiments at 39oC for 24 h. Total gas productionand methane emission were measured during and after the incubation, respectively. Results revealedthat, in general, addition of lacticin had limited significant effects on gas production parameterscompared to control (without lacticin addition). Lacticin addition up to 50 µM did not significantlydecrease CH4 emission, although a tendency of methane reduction existed when lacticin was added at 25µM. Hay diet produced significantly less methane emission than that of hay:concentrate diet both inexperiment 1 and experiment 2 (P<0.05). It can be concluded that lacticin addition up to 50 µM wasunable to decrease CH4 emission in vitro and therefore need to be tested further at higher concentrations.
Co-Authors Anuraga Jayanegara K. Becker