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All Journal Microbiology Indonesia BioEksakta : Jurnal Ilmiah Biologi Unsoed
ENDANG SRIMURNI KUSMINTARSIH
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Horizontal Transfer of the “Popcorn-Effect” Strain of Wolbachia from Drosophila melanogaster to Stomoxys calcitrans ENDANG SRIMURNI KUSMINTARSIH
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.23 KB) | DOI: 10.5454/mi.3.3.5

Abstract

The Wolbachia containing haemolymph from Wolbachia infected Drosophila third/fourth instar larvae was transferred through microinjection into 5-days-old pupae of the blood sucking stable fly, Stomoxys calcitrans. There was no previous record of Wolbachia being present in S. calcitrans. From a total of 682 emerging adults, 236 were females and of these, seven females were tested positive (approximately 3%) for Wolbachia infection. Using electron microscopy, it was shown that Wolbachia were present in the muscle tissues of S. calcitrans.
Reverse Transcriptase PCR (Rt-PCR) for Detection of Dengue and Chikungunya Virus of Mosquito Aedes aegypti in Sokaraja Dyah Retno Annisa; Endang Srimurni Kusmintarsih; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.337 KB) | DOI: 10.20884/1.bioe.2020.2.1.1811

Abstract

dengue fever (DF). Meanwhile, chikungunya virus causes Chikungunya fever (CF). These diseases involve three organisms, namely virus, mosquito Aedes sp., and human. The transmission of dengue and chikungunya virus is related to the population of Ae. aegypti. Banyumas regency is one of the regions with many cases of dengue and chikungnya virus infections, particularly in Purwokerto, Sokaraja, and Cilongok sub-district. Up to this time, there is no medicine and vaccine provided to treat these viruses effectively. Thus, detection of virus inside vector will be effectively performed in order to predict the transmission risk of dengue and chikungunya virus. This research aimed to know the molecular detection of dengue and chikungunya virus on adult Ae.aegypti mosquito in Sokaraja Region, Banyumas Regency. Survey was done by a cross-sectional method in Sokaraja sub-district from May 2019 – March 2019. Furthermore, technical sampling that used was purposive sampling method of adult Ae.aegypti using BG-Sentital Trap, followed by molecular detection of dengue virus using Two-step RT-PCR and chikungunya gene virus using RT-PCR. Molecular detection of DENV and CHIKV of mosquitoes which collected from Sokaraja region showed negative result
Co-Authors Dyah Retno Annisa Trisnowati Budi Ambarningrum