Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Microbiology Indonesia Annales Bogorienses
TIWIT WIDOWATI
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Studies for IAA (Indole-3-Acetic Acid) Production by Isolates H6 with Nitric Acid Mutation RAHAYU FITRIANI WANGSA PUTRIE; TIWIT WIDOWATI; HARMASTINI SUKIMAN
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.555 KB) | DOI: 10.5454/mi.11.1.3

Abstract

Nitric acid mutations are known could be used for strain improvement. This research aimed to studies IAA production by nitric acid mutan were compared with wild type. Mutation were conducted with some different treatment time such as 0, 30, 60, 90 and 120 min subsequently it were measured for IAA production. Isolate H6 as -1wild type isolates were also molecularly identified. The wild strain exhibited 53.83 µg mL of IAA while the -1 -1. nitric acid mutan within a range 77.39 µg mL to 95.70 µg mL Isolates H6.60 exhibited the highest IAA -1 production which 39.87 µg mL higher were compared with wild-type. Based on 16S rRNA gene analysis, isolate H6 belonged to Lysobacter sp. ES2-22.
POTENTIAL OF GROWTH HORMONES IAA OF ENDOPHITIC MICROBES FROM NEEM PLANTS FOR CAPITAL OF INDUSTRIAL OPPORTUNITY Sylvia J R Lekatompessy; Nuriyanah; Liseu Nurjanah; Eleazar Handoyo; Rumella Simarmata; Tiwit Widowati
Annales Bogorienses Vol. 25 No. 1 (2021)
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia has a tropical climate that is rich in diversity of microorganisms with useful potential and has important values that can be developed in the fields of industry, health and agriculture. Neem is a plant commonly used as an insecticide for pest control. Biotechnology continues to develop in exploiting the unique and interesting potential of endophytic microbes to obtain useful bioactive compounds extracted from plants, without having to take large amounts of plant biomass. This study aims to explore other potentials of endophytic microbes from neem plants, namely as a producer of IAA growth hormone to support plant growth. The results of the test of growth hormone-producing microbes, from 19 isolates of endophytic bacteria tested, obtained 3 isolates of IAA hormone-producing bacteria. 36 isolates of endophytic fungi were tested, 18 isolates of endophytic fungi were positive for IAA hormone production. One of the isolates of endophytic fungi that produces the highest IAA hormone, has been identified molecularly and the result is Colletotrichum gloeosporioides. The potential value derived from the endophytic microbes of neem plants can be developed into biotechnology products that have high commercial value by utilizing potential genes to support plant growth.
Co-Authors Eleazar Handoyo HARMASTINI SUKIMAN Liseu Nurjanah Nuriyanah RAHAYU FITRIANI WANGSA PUTRIE Rumella Simarmata Sylvia J R Lekatompessy