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HANIES AMBARSARI
Badan Pengkajian Penerapan Teknologi (BPPT)
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Performance Optimization of Microbes from Shrimp Pond Sediment by Adding EM4 In Nitrification Process for the Treatment of Wastewater Containing High Ammonia Concentration HANIES AMBARSARI; MUHAMMAD RAHMADI HARAHAP
Microbiology Indonesia Vol. 11 No. 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1440.492 KB) | DOI: 10.5454/mi.11.3.4

Abstract

 In liquid wastes, especially domestic wastewater, many organic substances are mixed causing water quality degradation, one of them is ammonia. Liquid wastes containing ammonia can be treated using an activated sludge system. One of the active sludge that can be used is shrimp pond sediments. This experiment investigated the performance of microbes in shrimp pond sediments with the addition of EM4 in nitrification process for the treatment of wastewater with high ammonia concentration in a 8 L batch reactor capacity. The results show that the addition of shrimp pond sediment as the active sludge can remove high ammonia level almost completely and there is known interaction between time and variation of shrimp pond quantity (p value <0,05) to the decreasing of ammonia level. Efficiency of decreasing the concentration of ammonia up to 100% can be reached on the 15th day in each treatment. The addition of EM4 can shorten the decreasing of ammonia level by 50%. Keywords: Nitrification, Ammonia, Shrimp Pond Sediment, EM4, Activated Sludge
Isolation and Urease Activity Test of Bacteria for Calcium Carbonate (Calcite) Precipitation (Biocementation) in Soil HANIES AMBARSARI; AFLAKHUR RIDLO
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (605.545 KB) | DOI: 10.5454/mi.12.3.2

Abstract

The use of bacterial calcium carbonate (calcite) precipitation (biocementation) has recently become popular as a ground-improvement technique. Ureolytic bacteria having highly urease activities were known to have important roles in calcium carbonate precipitation process. One of our research objectives is to isolate and to select as many as possible such ureolytic bacteria from Indonesian soils to be further utilized for calcium carbonate (calcite) precipitation process in the soil for strengthening the soil structure. Isolation was performed anaerobically in selective media containing 40% urea. Four isolates with different morphologies were purified and coded as TK1, TK2, TK3, and TK4. Each of them was tested for its urease activity either as a pure culture or as a mixture of several cultures. The urease activity was measured based on the ammonia concentration produced in the growth media up to 7 x 24 hours. It was known that isolate TK4 had the highest urease activity on week 6, whilst a mixture of isolate cultures coded as TKC did not show a better urease activity than the isolate TK4. Hence, it could be concluded that the isolate TK4 was the best candidate to be used for further research on the calcium carbonate (calcite) precipitation process (biocementation) to strengthen the soil structure.
Co-Authors AFLAKHUR RIDLO MUHAMMAD RAHMADI HARAHAP