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All Journal Microbiology Indonesia Annales Bogorienses
Anggia Prasetyoputri
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Antibacterial and Antioxidant Activities of Ginger Essential Oils Mega Ferdina Warsito; Febriana Untari; Anggia Prasetyoputri; Fauzy Rachman; Eris Septiana; Asep Bayu; Akhirta Atikana; Linda Sukmarini; Masteria Yunovilsa Putra
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1605.595 KB) | DOI: 10.5454/mi.15.4.1

Abstract

Ginger is a rhizomatous perennial herb that grows abundantly in tropical areas. It has been used around the world as a spice, flavoring agent, and ingredient in traditional medicine. Ginger essential oils (GEOs) are derivatives of ginger that can be found in various products used in daily life, such as food, pharmaceutical, and cosmetics. The present study analyzed the chemical compositions, antioxidant, and antibacterial activities of three commercially available GEOs. The compositions of GEOs were identified using the gas chromatography method. The antioxidant activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2’-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay methods. The antibacterial activity was determined using a disc diffusion assay based on the diameter of the inhibition zone (DIZ). The main compounds identified from the samples were zingiberene, α-curcumene, β-sesquiphellandrene, camphene, α-farnesene, β-bisabolene, α-pinene, and 3-carene. The IC50 values were found to be 5.3023 and 1.4504 mg/mL for GEO1; 0.9249 and 0.5276 mg/mL for GEO2; and 10.4463 and 3.3535 mg/mL for GEO3 when evaluated using DPPH and ABTS assay methods, respectively. All samples showed antibacterial activity against Staphylococcus aureus ATCC 13420 and Bacillus subtilis (collection of Indonesian Institute of Sciences), while only GEO2 and 3 displayed inhibitory effect against Escherichia coli ATCC 9637.
The Development of A Bioassay Based on Heterologous Expression of M2 Ion-Channel Protein Anggia Prasetyoputri; Neti Yuliaty; Warda Tuharea; Alisin Febyanti; Bambang Sunarko; Ines I. C. Atmosukarto
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2010.v14.n2.9-14

Abstract

Emerging resistant viral strains combined with the limited availability of antivirals in a pandemic scenario highlight the need for the development of novel influenza antivirals. A bioassay based on the M2 protein of influenza virus - a potential target for antivirals - was developed to screen endophytic microbial extracts. M2 can be synthesized using PCR, thus eliminating the need for the handling of infectious specimen. Following cloning of the M2 gene into a pET backbone, the resultant plasmid was transformed into BL21 (DE3) pLyss E. coli cells. Cultures of these cells were set up at 37C following inoculation with a starter culture, to reach an OD at 600nm (OD600) of 0.4-0.6. Once at the required OD, the culture was split in two aliquots and expression of the M2 protein was induced in one of the duplicates with the addition of isopropyl β-D-thiogalactopyranoside (IPTG). Bacterial growth was monitored at 60-minute intervals. Exogenous expression of the M2 protein has been reported to decrease host cells viability, resulting in lower OD600 values. Our results suggest that the M2 protein was expressed and that overexpression of this protein resulted in consistently lower OD600 values of induced cultures compared with that of uninduced cultures. Based on this principle, extracts can be screened for their ability to block M2 function as identified by increased OD600 values.
Enhancing the Immunogenicity of Subunit Vaccines by Utilisation of Particulate Vaccine Delivery Systems Anggia Prasetyoputri; Wien Kusharyoto
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2013.v17.n2.1-16

Abstract

Control and eradication of a number of infectious diseases are primarily attributed to effective vaccination programs. A concerted effort is still imperative to develop novel vaccines and improve the immunogenicity of existing ones with regards to efficacy, immunogenicity and safety. Rational design of vaccines using subunit vaccines is a potentially safer alternative to conventional vaccines, yet they are poorly immunogenic without additional adjuvant. Using antigen carriers to enhance their immunogenicity in the forms of adsorption or encapsulation with a delivery system has been widely investigated as an alternative to currently available adjuvants. This review aims to elaborate on the existing nanotechnology being used to develop more immunogenic subunit vaccines, with focus on particulate delivery systems for development of prophylactic vaccine candidates. 
Co-Authors Akhirta Atikana Alisin Febyanti Asep Bayu Bambang Sunarko Eris Septiana Fauzy Rachman Febriana Untari Ines I. C. Atmosukarto Linda Sukmarini Masteria Yunovilsa Putra Mega Ferdina Warsito Neti Yuliaty Warda Tuharea Wien Kusharyoto