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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Nandang Suharna
Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Cibinong, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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RECOVERY OF MORE THAN 10 YEARS-DRYING Monascus CULTURES AND ITS PURIFICATION METHODS FROM FUNGAL AND BACTERIAL CONTAMINATION Nandang Suharna
BIOTROPIA Vol. 15 No. 1 (2008): BIOTROPIA Vol. 15 No. 1 June 2008
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.553 KB) | DOI: 10.11598/btb.2008.15.1.7

Abstract

This study was carried out to understand the recovery capability of more than 10 years- drying Monascus cultures. A new simple purification technique from fungal contamination using ethanol-soaking treatment was also reported as a part of this study. The result showed that all drying cultures were recovered well and retained their characters such as good growth, pigmen­tation and production of fruit bodies (ascomata), sexual spores (ascospores) and asexual spores. Several cultures showed its good growth in 20% ethanol medium. This study also reported suc­cessful purification of cultures from fungal contamination using ethanol-soaking treatment. This self-drying method, therefore, could be suggested as a good long-term preservation method for Monascus cultures. Moreover, purification method from fungal contamination soaked in ethanol 70% or 95% was successfully effective.
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