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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Agus Nuryanto
Fac. of Biology Jenderal Soedirman University Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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GENETIC VARIABILITY OF POLYMESODA EROSA POPULATION IN THE SEGARA ANAKAN CILACAP Agus Nuryanto; AGUS HERY SUSANTO
BIOTROPIA Vol. 17 No. 1 (2010): BIOTROPIA Vol. 17 No. 1 June 2010
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1085.718 KB) | DOI: 10.11598/btb.2010.17.1.37

Abstract

Abstract Mud clams Polymesoda erosa in the Segara Anakan Cilacap are highly exploited by the local communities for daily consumption. This is presumed causing population decline and potentially causing loss of genetic diversity. Genetic diversity level within population can be obtained by population genetic study using molecular marker such as randomly amplified polymorphic DNA (RAPD). Here we amplified RAPD marker using ten arbitrary primers to assess genetic diversity of P. erosa population in the Segara Anakan Cilacap to provide genetic data for its sustainable use. The result proved that the used RAPD marker has high polymorphisms. The mud clam population was also showed a high level of heterozigosity and genetic diversity.  This has important implication for the management plan towards ustainable use of P. erosa in the Segara Anakan Cilacap.   Key words: mud clam, RAPD, polymorphism, genetic diversity
MOLECULAR IDENTIFICATION AND PHYLOGENETIC RELATIONSHIP AMONG LOCAL, SANGKURIANG, AND AFRICAN CATFISH BASED ON RAPD MARKER Agus Nuryanto
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13132.152 KB) | DOI: 10.11598/btb.2012.19.1.49

Abstract

Catfish species are commercially cultivated freshwater fish by fish farmer. Formerly, only one species of catfish had been cultivated, namely local catfish (walking catfish) Clarias batrachus.  However, since 1985, local catfish was replaced by a new commodity from Africa called African catfish (C. gariepinus).  In 2004, BBPBAT Sukabumi had introduced a new strain of catfish, namely Sangkuriang catfish.  Taxonomic analysis needed, especially for Sangkuriang catfish using molecular marker, in order to get a clearer picture about its genetics departure from their parental, so their taxonomic status and relationships with local and African catfish is clearer.  One of the molecular markers is randomly amplified polymorphic DNA (RAPD) markers. Species status defined based on the presence and absence of specific band on each catfish.  Phylogenetics relationships was inferred from phylogenetic tree which was built using UPGMA tool as implemented in NTSYS software based on the similarity of RAPD band pattern. The result showed that Sangkuriang and local catfish had specific RAPD markers, while African catfish was not. This means that all RAPD markers of African catfish were shared with those of two others catfish. Local catfish phylogenetically distantly related to African and Sakuriang catfish. Only one individual of African catfish (D4) genetically close related to Sangkuriang catfish while the rest individuals of African catfish genetically closed related to green catfish. This means that African catfish showed very divergence genetic constituent.    Key words: catfish, molecular identification, phylogenetic relationships, RAPD
Co-Authors AGUS HERY SUSANTO