Fusarium is one of the important genera associated with grasses as saprophytes endophytesand pathogens A study was carried out on distribution and diversity of Fusarium speciesassociated with two groups of grasses in 10 states throughout Peninsular Malaysia ieagricultural grasses Oryza sativa and Saccharum officinarum and nonagricultural grassesAxonopus compressus Centhotheca lappacea Chloris barbata Crysopogon aciculatus Cyanadon dactylonDactyloctenium aegyptium Digitaria ciliaris Echinochloa colona Eleusine indica Eragrostis amabilisEragrostis malayana Eragrostis uniloides Ischaemum magnum Panicum brevifolium Panicum millaneumPanicum repens Paspalum commersonii Paspalum conjugatum Paspalum orbiculare Pennisetum purpureumSacciolepis indica Sporobolus diander and Sporobolus indicus A total of 474 isolates were singlespored and identified by morphological characteristics F semitectum was frequently isolated236 followed by F sacchari and F fujikuroi with 154 and 146 respectively The othernine species were F solani 103 F proliferatum 89 F oxysporum 74 F subglutinans65 F equiseti 55 F verticillioides 34 F compactum 25 F chlamydosporum 11 andF longipes 08 Based on the ShannonWeiner Index F solani was the highest H 262isolated from grasses Species of Fusarium from O sativa were widely diverse with 11 speciesfollowed by nonagricultural grasses with nine species and S officinarum with only six speciesThis is the first report on diversity of Fusarium associated with grasses in MalaysiaKey words Oryza sativa Saccharum officinarum nonagricultural grass Fusarium speciesdiversity and Gramineae