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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Berkala Penelitian Hayati
Iman Hidayat
Microbiology Division, Research Center for Biology-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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CHARACTERISTICS OF THE PEAT SOIL Bacilllus sp. Iman Hidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/375

Abstract

Twenty two strains of Bacillus species have been isolated from the peat soil of Tanjung Puting, Central Kalimantan. All strains devided into seventeen groups which characterized using morphology and biochemical tests. All strains have been tested for lipase, amylase, protease, cellulase, and phosphatase activity. Thirteen Bacillus isolates tested gave positif reaction for lipase activity. Bacillus AR 018 was the best isolate for amylase activity, Bacillus AR 001 and Bacillus AR 002 for protease activity, Bacillus AR 009 for cellulase activity. Only one isolate shows positive reaction for phosphatase activity, notably Bacillus AR 007.
A SPECIES-SPECIFIC PCR ASSAY BASED ON THE INTERNAL TRANSCRIBED SPACER (ITS) REGIONS FOR IDENTIFICATION OF MYCOSPHAERELLA EUMUSAE, M. FIJIENSIS AND M. MUSICOLA ON BANANA Iman Hidayat
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.716 KB) | DOI: 10.11598/btb.2012.19.1.232

Abstract

A study on development of a rapid PCR-based detection method based on ITS region of M. eumusae, M. fijiensis, and M. musicola on banana was carried out. The main objecive of this study was to develop a fast and species-specific PCR-based detection method for the presence of Mycosphaerella species on banana. The methods include collection of specimens, morphological identification supported by molecular phylogenetic analysis, RFLP analysis, species-specific primers development, and validation. Two species of Mycosphaerella, namely, M. fijiensis and M. musicola, and one unidentified Pseudocercospora species were found in Java island. Three restriction enzymes used in the RFLP analysis, viz, AluI, HaeIII, and TaqI were capable to discriminate M. eumusae, M. fijiensis, and M. musicola. Two species-specific primer pairs, viz, MfijF/MfijR and MmusF/MmusR have been successfully developed to detect for the presence of M. fijiensis and M. musicola, respectively.
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