Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI
I Made Sudiana
Research Center for Biology-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2207

Abstract

The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungi  Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed  on Saccharomycopsis fibuliger.  The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.  Optimum temperature and pH for enzyme production were 30°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.
LIPID ACCUMULATION BY Flavodon flavus ATH USING PALM OIL MILL EFFLUENT AS SUBSTRATE I Made Sudiana; Atit Kanti; Helbert Helbert; Senlie Octaviana; Suprapedi Suprapedi
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.989 KB) | DOI: 10.11598/btb.2014.21.2.388

Abstract

Large amount of palm mill effluent is generated annually. The waste would be potential for production of single cell oils (SCOs). The objective of study was to evaluate the lipid accumulation by fungi using palm mill effluent as substrate. To obtain most potential strains for lipid accumulation, seven filamentous fungi isolated from various biomes were evaluated for their ability to produce endoglucanase, and its lipid accumulation. Fungal hypae grown on palm oil mill effluent accumulated lipid of 34,3-87,5 of their dry cell mass. The profile of transesterified SCOs revealed a high content of saturated and monounsaturated fatty acids i.e., palmitic (C16:0), stearic (C18:0) and oleic (C18:1) acids similar to conventional vegetable oils used for biodiesel production. The strain was able to use organic substrates in POME implies that they are promising strain for biofuel feed stock as well as for meeting effluent quality for wastewater discharge.
Co-Authors Atit Kanti Atit Kanti Helbert Helbert Senlie Octaviana Suprapedi Suprapedi