Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Kefarmasian Indonesia Parapemikir Jurnal Ilmiah Farmasi
Hendig Winarno
Universitas Pancasila
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Isolasi dan Identifi kasi Senyawa Kimia Zat Anti Kanker dari Daun Kopasanda (Chromolaena odorata (L.)) MUHAMMAD FITRAH; HENDIG WINARNO; PARTOMUAN SIMANJUNTAK
JURNAL ILMU KEFARMASIAN INDONESIA Vol 15 No 1 (2017): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1911.218 KB)

Abstract

Isolation and identifi cation of chemical compound as anticancer from the leaves of Kopasanda (Chromolaena odorata L.) had been done. Extraction was conducted successfully with n-hexane, ethylacetate and ethanol by maceration, and by refl ux for water solvent. Fractionation of ethylacetate extract by column chromatography (SiO2). n-hexane-ethylacetate = 10:1 ~ 1:1; ethylacetate). n-hexaneethylacetate = 5:1) gave one pure isolated fraction fr. 5-3 with inhibition activity against leukemia cell line L1210 at concentration 1.515 ppm. Chemical structure determination was done based on spectral data interpretation of Infra Red, 1D NMR (1H, 13C) and 2D NMR (H-H COSY, HMQC and HMBC) and mass spectra (LC-MS). Pure isolated fraction fr. 5-3 was deducted as methyl ether naragenine.
Aktivitas Sitotoksik dan Profil Kromatogram Daun Sirsak (Annona muricata L.) yang Diiradiasi ERMIN KATRIN; RHILA AMALIAH; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1306.595 KB)

Abstract

Soursop (Annona muricata L.) is one of plants included in Annonaceae which the leaves has cytotoxic activity on cancer cells. Preservation efforts of soursop leaves is carried out by gamma irradiation technic. This research aimed to study the effect of gamma irradiation on the bioactivity and chromatogram profiles of the active fraction from soursop leaves.Dry powders of soursop leaves were irradiated using gamma with doses of 5; 7.5; 10; and 15 kGy and repeated twice, respectively for certain doses. Then each sample was macerated with n-hexane, ethyl acetate and ethanol gradiently. Each extract was tested against leukemia L1210 cells. Ethyl acetate extract was the most active (IC50= 7.36 µg/mL) compared with n-hexane (20.18 µg/mL) and ethanol extract (13.89 µg/mL). Ethyl acetate extracts was fractionated using column chromatography obtained ten fractions. The result of cytotoxic activities assay of ten fractions were obtained that fraction 8 was the most active with IC50 value of 0.45 µg/mL. Cytotoxic activity of fraction 8 from irradiated samples showed that gamma irradiation decreased cytotoxic activity of fraction 8, but they were still active (IC50 < 20 µg/mL). Analysis and identification of fraction 8 by the HPLC and TLC-densitometry showed that there were broad peak which decreased in the irradiated samples.The maximum radiation dose for soursop leaves without damaging its bioactivity and that did not change the chromatogram profilesis 7.5 kGy.
Iradiasi Sediaan Obat Herbal Temu Putih Curcuma zedoaria (Berg) Rosc.: Cemaran Mikroba, Sitotoksisitas dan Profil Kromatogram ERMIN KATRIN W.; EPSI NARULITA; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.715 KB)

Abstract

Gamma irradiation has been used for preservation of herbal medicines, one of which is temu putih Curcuma zedoaria (Berg) Rosc. to cure cervical cancer. This research aimed to study the gamma irradiation effects on microbial contamination, cytotoxic activity against leukemia L1210 cell lines and chromatogram profile of the temu putih active fraction. Gamma irradiation was performed with a 60Co source at 5, 7.5, 10 and 15 kGy. After irradiation at > 7.5 kGy bacteria and mould were not present on herbs. Control and irradiated samples were consecutively macerated with n-hexane, ethyl acetate and ethanol. Ethyl acetate extract was the most cytotoxic gainst L1210 leukemia cells with an IC50 value of 10.60 μg/mL as compared to n-hexane or ethanol extract. Ethyl acetate extract was fractionated with column chromatography producing six fractions. Fraction 2 was the most cytotoxic against L1210 leukemia cells with an IC50 value of 2.44 μg/mL. The thin-layer chromatographic analysis results of ethyl acetate extract and fraction 2 of control and irradiated samples showed the presence of curcumin and chromatographic pattern similar to the control. Curcumin contents in fraction 2 were between 35 to 51%, which were not significantly changed between samples, although was irradiated up to 15 kGy. The maximum dose for irradiation of temu putih herbal preparation is 15kGy.
Penentuan Kadar Fenol Total Dan Uji Aktivitas Antioksidan Kombinasi Ekstrak Herba Pegagan (Centella asiatica L. Urban) Dan Buah Mahkota Dewa (Phaleria macrocarpa(Scheff.) Boerl.) Purgiyanti Purgiyanti; Anny Victor Purba; Hendig Winarno
Parapemikir : Jurnal Ilmiah Farmasi Vol 8, No 2 (2019): Parapemikir : Jurnal Ilmiah Farmasi
Publisher : Pusat Penelitian dan Pengabdian Masyarakat Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/pjif.v8i2.1395

Abstract

Many diseases that were originally caused by free radicals, so that free radicals and antioxidants observed extensively in the world of health. Gotu kola herb and fruit Mahkota Dewa were supposed to have antioxidant activity that can reduce the activity of free radicals. The purpose of this study was to determinethe total phenol content and the antioxidant activity of the combination of herbal extracts of Gotu kola (Centella asiatica L. Urban) and Mahkota Dewa fruit (Phaleria macrocarpa (Scheff.) Boerl.) Gotu kola herb extracts and Mahkota Dewa fruit extracts obtained by maceration using ethanol 70% with the ratio between crude drug powder and solvent was 1: 5. The antioxidant activity was determined by DPPH (2,2-diphenyl-1 picrylhydrazyl). Total phenol content was determined using UV-Vis spectrophotometry reagent Folin- Ciocalteau the results expressed in gallic acid equivalents (mg GAE / 100 g extract). The result shows that the combination of the ethanol extract of Centellaasiatica herb and Mahkota Dewa fruit in the ratio of 1: 1 has a total phenol content of 3.67 mg GAE / 100 g of extract and active as an antioxidant with IC50 value of 67.07 mg / mL, and vitamin C as active comparison as an antioxidant with IC50 value of 8.81 mg / mL. This study shows that a combination of herbal extracts between Gotu Kola and MahkotaDewa have potentials as natural antioxidants.Keywords— Gotu Kola herb, Mahkota Dewa fruit, total phenols, Folin-Ciocalteau,DPPH
Co-Authors Anny Victor Purba EPSI NARULITA ERMIN KATRIN ERMIN KATRIN W. MUHAMMAD FITRAH Purgiyanti Purgiyanti RHILA AMALIAH Simanjuntak, Partomuan Zuhelmi Aziz