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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Teknologi Pertanian
Y. Marsono
Fakultas Teknologi Pertanian, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT

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Hypoglycemic Effect Determination of Soybean, Soybean Protein Fraction and Tempe in Diabetic Rats Wulan, Siti Narsito; Astuti, Mary; Marsono, Y.; Noor, Zuheid
Jurnal Teknologi Pertanian Vol 3, No 2 (2002)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.93 KB)

Abstract

Hypoglicemic effect of soybean, soybean protein fraction and tempe was determined in alloxan-induced diabetic rats.  Blood glucose and urinary glucose level were measured to determine this effect.Soybean protein fraction had the highest hypoglicemic property.  It lowered blood glucose level from 37.35 to 5.48 mg/dl/day/g carbohydrate consumed (85%).  Tempe showed lower hypoglycemic effect than soybean  protein fraction.  Blood glucose level decreased 51%, from 31.57 to 15.59 mg/dl/day/g carbohydrate consumed.  Soybean had the lowest hypoglycemic effect.  It lowered blood glucose level from 47.47 to 27.70 mg/dl/day/g carbohydrate consumed (42%).  Urinary glucose level had the same pattern as blood glucose level.Keywords :  Hypoglicemic effect, blood glucose level, soybean, tempe
Indeks Glisemik Kacang Buncis (Phaseolus vulgaris) dan Kacang Panjang (Vigna sesquipedalis) serta Uji Efek Hipoglisemiknya pada Tikus Sprague Dawley Marsono, Y.; Triwitono, Priyanto; Kanoni, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.813 KB) | DOI: 10.24002/biota.v10i3.2879

Abstract

Glycemic index (GI) of foods has been suggested as an additional parameter in selecting foods for the diabetic patients in nutritional management. GI was measured as the area under the postprandial blood glucose curve for a food expressed as a percentage of the area after the consumption of reference food (bread). In this research two legumes were investigated including snap bean (Phaseolus vulgaris L.) and yardlong bean (Vigna sesquipedalis). The objectives of this research were (i) to determine the IG of the beans and (ii) to investigate the effects of these legumes on serum glucose concentration of alloxan-induced diabetic rats. Ten health and normal volunteers (not diabetic) were provided. The volunteers took an overnight fasting, blood were drawn in the morning and analyzed for serum glucose. Then they were given the test beans containing total carbohydrates equivalent to 25-g glucose to be consumed. Blood samples were drawn for glucose measurement every 30 minutes until 120 min after meal. Serum glucose was determined enzymatically and the glucose responses were drawn graphically to calculate the GI of the beans. These beans were evaluated for their hypoglycemic properties in alloxan-induced diabetic rats. They were fed modified AIN 93 diets contain 20% of energy derived from snap bean or yardlong bean for 28 days. Serum glucose concentrations were measured weekly. It was found that with white bread as the food standard the GI of the snap bean and yard long bean were 43 and 86, respectively. In 28 days of diet intervention, snap bean decreased the serum glucose level by 73% (from 212mg/dL to 56mg/dL). Similar effect were found for the yardlong bean. This bean can reduced serum glucose level about 73% (from 224mg/dL top 61mg/dL), but both were similar to control diet that decreased the serum glucose level about 72% .
Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Murdiati, Agnes; Wuryastuty, Hastari; Marsono, Y.; Harmayani, Eni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.509 KB) | DOI: 10.24002/biota.v14i3.2576

Abstract

Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.
Co-Authors Agnes Murdiati Eni Harmayani Hastari Wuryastuty Kanoni, Sri Mary Astuti Siti Narsito Wulan Triwitono, Priyanto Zuheid Noor