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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Tumbuhan Obat Indonesia Biotropika Research Journal of Life Science Natural B Berkala Penelitian Hayati
Retno Mastuti
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : natural b

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Effect of Nitrogen Fertilizer on Calcium Oxalate Calibration Density of Porang Bulb (Amorphophallus muelleri Blume) Bina Rizki Amalia; Nunung Harijati; Retno Mastuti
Natural B, Journal of Health and Environmental Sciences Vol 2, No 3 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (703.97 KB) | DOI: 10.21776/ub.natural-b.2014.002.03.11

Abstract

The aim of this research was to know the effects of nitrogen fertilizer to the shape and density of calcium oxalate (CaOx) crystals in porang tuber. Bulbil derived from porang plant in the first growth period seeded until the leaves fully open. Seedlings were transferred to a polybag after most of them has height 50 cm. After adapting for three weeks, the plants were treated with nitrogen fertilizer (urea) with a dose 0, 1, and 5 g/polybag. The plants were grown until the end of the growing period. The slides were prepared by slicing tuber as thin as possible using a sliding microtome. Sliced tuber of porang were cleared by using the modified clearing method. The slides were observed using light microscope to calculate the density of CaOx crystals. The densities of calcium oxalate crystals were analyzed using ANOVA (α = 0,05). The results showed that porang tuber has four kind of crystals i.e. raphide, druse, stiloid, and prism. Nitrogen fertilization had no significant effect on the densities of calcium oxalate crystals.
Co-Authors Bina Rizki Amalia Budi Waluyo Edia Fitri Dwinianti Estri Laras Arumingtyas Hiroshi Miyake Jati Batoro Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nurul Chairiyah Nurul Chairiyah Takeshi Taniguchi Wahyu Widoretno Yoji Takeoka