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All Journal Jurnal Tumbuhan Obat Indonesia PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia)
Lilik Nurfadlilah
Stikes Rumah Sakit Anwar Medika
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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BIOTRANSFORMASI KANDUNGAN SENYAWA KIMIA MINYAK GURJUN BALSAM MENGGUNAKAN Aspergillus niger Khoirun Nisyak; A'yunil Hisbiyah; Lilik Nurfadlilah
Jurnal Tumbuhan Obat Indonesia Vol 14 No 2 (2021): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v14i2.4778

Abstract

ABSTRACT Gurjun balsam oil is one of the essential oils from Indonesia isolated from Dipterocarpus turbinatus resin. Gurjun balsam oil has a fragrant aroma and benefits as a traditional medicine in the Indochina region. The main chemical content of balsam gurjun oil is alfa-copaena and several other sesquiterpenes (C15H24) class compounds. In this study, biotransformation of the gurjun balsam oil with Aspergillus niger was carried out at room temperature with an agitation speed of 130 rpm and avariation of the incubation time of 24, 48, 72, and 96 hours. The biotransformation of balsam gurjun oil produced some main products as follows: alfa-copaene (60.53%), beta-caryophyllene (22.76%), humulene (3.87%), and alpha-cadinene (12.83%). The optimum incubation time with the highest copaena product was 72 hours. Biotransformation of gurjun balsam oil by Aspergillus niger does not produce new derivatives but increases the yield of the alpha-copaena. Αlpha-copaena in gurjun balsam oil has strong potential as anti-eczematic in treating skin problems. Keywords: biotransformation, gurjun balsam oil, alpha-copaena, and Aspergillus niger ABSTRAK Minyak gurjun balsam merupakan salah satu minyak atsiri asal Indonesia yang diisolasi dari resin tanaman Dipterocarpus turbinatus. Minyak gurjun balsam memiliki aroma yang harum dan digunakan sebagai obat tradisional di daerah Indocina. Kandungan kimia utama minyak balsam gurjun adalah alfa-kopaena dan beberapa senyawa golongan seskuiterpen (C15H24) lainnya. Pada penelitian ini dilakukan biotransformasi minyak gurjun balsam dengan Aspergillus niger. Proses biotransformasi dilakukan pada suhu kamar dengan kecepatan agitasi 130 rpm dan variasi waktu inkubasi 24, 48, 72, dan 96 jam. Produk yang ditransformasi kemudian dianalisis dengan Gas Chromatography-Mass Spectrometer (GC-MS). Produk utama yang dihasilkan dari biotransformasi minyak balsam gurjun adalah alfa-kopaena (60,53%), beta-karyofilena (22,76%), humulena (3,87%), dan alfa-kadinena (12,83%). Waktu inkubasi optimum dengan produk kopaene tertinggi adalah 72 jam. Biotransformasi minyak gurjun balsam oleh Aspergillus niger tidak menghasilkan turunan baru tetapi meningkatkan rendemen alfa-kopaena. Alfa kopaena memiliki potensi sebagai anti eksim yang kuat untuk mengatasi masalah kulit. Kata kunci: biotransformasi, minyak gurjun balsam, alfa-copaena, dan Aspergillus niger
Method Validation of Silica Dispersive Solid Phase Extraction Combined with Spectrophotometer UV-Vis for the Determination of Allopurinol in Herbal Medicine Eviomitta Rizki Amanda; Anisa Suci Rosmawati; Lilik Nurfadlilah; Gama Prakoso Buono; Yani Ambari
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), Vol. 19 No. 01 Juli 2022
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v19i1.10219

Abstract

A facile sample preparation method based on silica dispersive solid-phase extraction combination with spectrophotometer UV-Vis for the extraction of allopurinol in herbal medicine was successfully developed. Silica was used as a solid sorbent. The extraction process was carried out by inserting and dispersing silicas in a 30 mL sample solution that contained allopurinol, then stirred using a hot plate stirrer. At the end of the extraction process, silicas were collected and desorbed using ethanol by utilizing a vortex. The desorption solution was analyzed by spectrophotometer UV-Vis at a maximum wavelength of 250 nm. Several essential parameters such as silica mass, extraction time, desorption time, and pH of sample solution were optimized. The results showed that the optimum extraction condition was achieved: silica mass. 0.8 grams; extraction time, 45 minutes; desorption time, 2 minutes; and pH of sample solution, pH 7. The optimum extraction condition was then applied for the standard curve and analyzed of allopurinol in herbal medicine samples. The results of the method validation method were obtained the correlation coefficient (R2), 0.9961; the detection limit, 0.6871 ppm; the quantitation limit, 2.2902 ppm, the percent of recovery (% R) in the range of 96.42-110.25%, percent coefficient of variation (% CV) in the range of 0.0361- 0.1322%. The application method in 3 real samples showed that the concentrations of allopurinol were 56.0221 ppm, 54.8706 ppm, and 63.6719 ppm, respectively. The values of % R in the analysis of real samples by using the spiking method were obtained in the range of 49.52-89.74%.
Co-Authors A'yunil Hisbiyah Anisa Suci Rosmawati Eviomitta Rizki Amanda Gama Prakoso Buono Nisyak, Khoirun Yani Ambari