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. Marwoto
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology

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Skrining Methicillin Resistant Staphylococcus aureus (MRSA) pada Tenaga Kesehatan Bangsal Melati 1, Melati 2, dan Mawar 2 RSUD Dr. Moewardi Sulaiman, Achmad Faiz; Marwoto, .; Purwoko, .
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: MRSA is known as one of main cause of nosocomial infection since found in 1961. In Indonesia, its prevalence reached 23,5%. Direct contact to healthcare workers can cause transmission of Staphylococcus aureus to patients. Carrier of chronic MRSA plays important roles on MRSA infection in hospital setting. This research aimed to know the incidence of MRSA nasal carriage of healthcare workers in RSUD Dr Moewardi Surakarta. Methods: This was an descriptive study using cross sectional design. Samples were taken from nasal swab of healtcare workers in wards of Melati 1, Melati 2 and Mawar 2. Samples were examined in Microbiology Laboratory of FK UNS. Total samples obtained were 74 according to total sampling theory. Data were analyzed descriptively and tested with Chi-Square tests. Results: The result of nasal swab from 26 healthcare workers in Melati 1 ward showed that there were 7 healthcare workers that were colonized by Staphylococcus aureus and one of them was MRSA case. In Melati 2 ward, from 25 healthcare workers, ten of them were colonized by Staphylococcus aureus and 8 out of 10 healthcare workers were MRSA cases. Whereas in Mawar 2 ward from nasal swab of 23 healthcare workers, six of them were colonized by Staphylococcus aureus and no MRSA case was found. Conclusions: The number of MRSA incidence in Melati 1, Melati 2 and Mawar 2 wards  consecutively were 1.33%, 10.67% and 0%. Keywords: MRSA, healthcare worker, colonization.
Uji Efektivitas Prosedur Disinfeksi Tingkat Tinggi Endoskopi Saluran Cerna RSUD Dr. Moewardi Ramadhani, Aviaddina; Marwoto, .; Saptawati, Leli
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: The risk of infection associated with gastrointestinal endoscope remains a topic of interest. This vexation may be related to reliability of the disinfecting techniques or the compliances with the guideline laid down for high-level disinfection. Gastrointestinal endoscope disinfecting techniques may vary from site to site. This study was to examine the effectiveness of gastrointestinal endoscope high-level disinfection at Rumah Sakit Umum Daerah (RSUD) Dr. Moewardi. Methods: An observational study using cross sectional design was conducted at RSUD Dr. Moewardi. Thirty samples from gastrointestinal endoscope swab after high-level disinfection process with 24 hours storage and without storage was sampled with convenience sampling technique. Data from this research were analyzed descriptively and tested using Chi square test. Results: Microorganism were detected in 11/14 samples of gastrointestinal endoscope after 24 hours storage and 3/16 samples of gastrointestinal endoscope without storage process. The microorganisms were Bacillus sp, Staphylococcus aureus, Staphylococcus non-aureus, and Clostridium sp. Significant relationship was detected between storage processes after gastrointestinal endoscope high-level disinfection with microorganism contamination. Conclusions: High-level disinfection of gastrointestinal endoscopes RSUD Dr. Moewardi was not yet effective. There was a significant relationship between gastrointestinal endoscopy storage for 24 hours with the effectiveness of high-level disinfection of gastrointestinal endoscopes. Keywords: high-level disinfection, gastrointestinal endoscope, 24 hours storage, without 24 hours storage.
Perbandingan Efektivitas Handrub Softa-Man® Dan Formula Handrub Moewardi Terhadap Angka Kuman Di RSUD Dr. Moewardi Saptawati, Leli; Marwoto, .; Jeffrey F .L, Henrikus
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Nosocomial infections are important clinical problem, indicated by many cases on the incidence of nosocomial infections. To overcome this incident, a lot of researchers have conducted variety of ways, one of them is hand hygiene. Two different kinds of hand hygiene alcohol-based products were used in this study, handrub Softa-man® and Moewardi’s handrub formula. The effectiveness of both handrub in reducing the number of bacteria was compared on health-care workers. Methods: This study was a quasi-experimental study. The study was conducted at hospital wards in Dr. Moewardi Hospital. Respondents were taken by consecutive sampling. There were two groups in this study, the group that received handrub Softa-man® and the group that received Moewardi’s handrub formula. Each group was observed for the effectiveness in reducing the number of bacteria. The data that had been collected then were analyzed using the Mann-Whitney test and followed by Wilcoxon test. Results: The results of this study showed that both groups managed to reduce the number of bacteria. Wilcoxon test result showed a difference in the number of bacteria before and after hand washing where the number of bacteria after hand washing was fewer than before hand washing. The result of Mann-Whitney test showed that there was no statistical difference in the decreased number of bacteria when both handrub were compared. Conclusions: There was no significant difference in the decreased number of bacteria between the Softa-man® handrub and Moewardi’s handrub formula usage. Keywords: Handrub’s effectiveness, Alcohol-based handrub, Softa-man®, Moewardi’s formula, Number of bacteria.
Co-Authors . Purwoko Achmad Faiz Sulaiman Aviaddina Ramadhani Henrikus Jeffrey F .L Leli Saptawati