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Alfian Siswanto
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Agriculture, Biological Sciences & Forestry

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BIODEGRADATION OF CACAO (Theobroma cacao L.) RIND WASTE BY Aspergillus niger WITH VARIATION OF INOCULUM CONCENTRATION AND INCUBATION TIME Alfian Siswanto; Ida Bagus Gede Darmayasa; I Ketut Muksin
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.172 KB) | DOI: 10.24843/JSIMBIOSIS.2020.v08.i01.p06

Abstract

Crop waste or cacao (Theobroma cacao L.) processing waste that doesn’t handle with care can polluting the environment around the farm. Rind of cacao can be used for composting, but it can be less effective because the nutrient of fresh cacao rind too complex and the ratio of C/N too high. This research intention to knows the potential of Aspergillus niger to degrade cellulose on waste cacao rind who have high ratio of C/N, with combination inoculum concentration and incubation time. The methods of this research begin with isolation and identification of A. niger from litter of cacao field, then the in vitro test done by CMCA (Carboxyl Methyl Cellulose Agar) selective media, and then the cacao rind was testing by in vivo method. The results of this research shown that A. niger can degrade cellulose by in vitro ways, it can be seen by the clear zone that formed on CMCA selective media. The in vivo test on cacao rind shown decreasing of C/N ratio between 18,940%-40,623%. Combination of A3B3 treatment (15 mL inoculum concentration, 28 days incubation time) indicate the lowest C/N ratio 18,940%.
Co-Authors I KETUT MUKSIN Ida Bagus Gede Darmayasa