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Djadja Siti Hazar Hoesen
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PERTUMBUHAN DAN PERKEMBANGAN TUNAS KULTUR EMBRIO Caryota no Becc. Djadja Siti Hazar Hoesen
BERITA BIOLOGI Vol 4, No 1 (1997)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i1.1293

Abstract

Caryota no Becc,(fish-tailpalm) is considered vulnerable palm, Its population is declining from the habitat by destructive exploitation but also to some extent by local people who remove the edible apex or 'cabbage 'as a vegetable and also obtain sago from the pith of the trunk,so kill the tree.A though its biological characteristics have not been fully studied,this species is also potentially for ornamental plant Thus conservation measures should be taken to improve the status of this threatened species. Tissue culture techniques may provide ways either to propagate or to conserve itin-vitro. Naturally Caryota no Becc.palm fruit after 20years and they can germinate more than 3 months after sowing with low germination value.In this experiment,the tissue culture response of this species are studied using the explant of embryo from (relatively) young fruit (juvenility fruit).The effect ofBA and 2,4D on the shoot growth, root growth and calluses was evaluated, while the evaluation of the effect ofactifated charcoal was discussed. The satisfactory results have not been achieved.
KULTUR IN-VITRO EKSPLAN RIMPANG Zingiber zerumbet van aromaticum Val Djadja Siti Hazar Hoesen
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1061

Abstract

Zingiberaceae species Zingiber zerumbet var. aromaticum Val. usually propagated by vegetative part of the plant. Study on the proliferation of rhizome explants through in-vitro method were conducted on this species for propagation.The explants were cultured in MS (Murashige & Skoog) composition medium in normal and half strength concentration of macro elements, supplemented with micro elements and vitamins. Plant growth regulator (PGR) cytokinin (kinetin, adenine sulphate) were added in initiation stage.Benzyl adenine (BA), thidiazuron and 2,4D (dichlorophenoxy acetic acid) added in medium as treatments to induce shoot multiplication and adventitious shoot development or rooting plants.The media for induced multiple shoot and roots (plantlets) development, supplemented with PGR BA, thidiazuron, Indole butyric acid (D3A) as treatments and activated charcoal were added as antioxidant.In general, most of cultures were indicated have positively responds to PGR treatments. The ability of culture to produce multiple shoots and roots such as in medium with BAP (8 mg/1) + thidiazuron (0.1 mg/1) + IBA (1 mg/l)-indole butyric acid would give a better opportunity of rhizome explants proliferation and plantlets development of Z. zerumbet var. aromaticum.
PERBANYAKAN DAN PENYIMPANAN KULTUR SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.] DENGAN TEKNIK IN-VITRO Djadja Siti Hazar Hoesen
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1122

Abstract

Sambung nyawa [Gynura procumbens(Lour.)Merr.]is one of the traditional medicine sources.The plants are not cultivated intensively in the field. One of the efforts to maintain and to propagate this species is by multiplication through tissue culture method. Nodes explants were cultured in MS normal and half strength concentration macroelements, supplemented with microelements and vitamins; combination of cytokinin BA (2 mg/1), thidiazuron (0.01 mg/1 and 0.1 mg/1) and adenine sulphate (5 mg/1).Auxin (2,4D 0.5 mg/1) in combination were added in media as treatments and activated charcoal (2 g/1) as antioxidant. Young leaves explants were also cultured in the same basic medium (MS and 'A MS) in treatments with cytokinin (BA and thidiazuron).The results from nodes and young leaves explant indicated that the highest number of survival cultures were obtained from combination between BA (2 mg/1) and thidiazuron (0.01 mg/1) in MS normal strength basic medium.In acclimatization stage, 100% of plantlets survived and successfully transplanted to soil medium in the field; maintenance study indicated that subculture was prolonged until 52 weeks.
KULTUR JARINGAN KUNIR PUTIH (Kaempferia rotunda L.) Djadja Siti Hazar Hoesen
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1269

Abstract

Shoot cultures of Kaempferia rotunda L.were established from rhizome segment.In the 1st experimen these explants were planted on Gamborg/B5 medium that supplemented with BA concentrations were (0, 0.5, 1 and 2) mg/l and/or kinetine were (0, 2 and 4) mg/l.In 2nd experiment these explants planted in Murashige and Skoog medium (MS).This medium supplemented with BA concentrations (0, 2 and 4) mg/l and/or NAA (0 and 1) mg/l.The result in the 1st experiment showed that the best proliferated shoots was from the culture that supplemented with BA 1 mg/l and kinetine 4 mg/l, while in the 2nd experiment the best proliferated shoots was from the culture that supplemented with BA 4 mg/l and NAA 1 mg/l.These shoots were then subcultured on MS liquid medium supplemented with BA (5 mg/l) and MS agar medium was supplemented with BA (2 mg/l) + 2iP (0.5 mg/l) + thidiazuron (0.01 mg/l) + NAA (0.5 mg/l).The cultures could induce the shoots number and produce the morphological plantlets for acclimatization,and acclimatization successed on soil and compost mixed medium in ratio 1:1.
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