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Heddy Julistiono
Bidang Mikrobiologi, Pusat Penelitian Biologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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CEKAMAN OKSIDASI SEL KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARASETAMOL DAN ANTIOKSIDAN (+)-KATEKHIN Heddy Julistiono
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1889

Abstract

In order to more understand similarity of yeast Candida tropicalis with mammalian cells in analgesic drug paracetamol metabolism and toxicity, ability of yeast in the drug metabolism and oxidative response of cells treated with the drug and (+)-catechin was studied. In mammalian cells, paracetamol toxicity is mainly caused by metabolite byproduct of drug metabolism catalyzed by cytochrome P450, a membrane-bound enzyme and peroxidase and a soluble enzyme. Previously it has been shown that paracetamol induced oxidative stress in the yeast cells; and green tea extract protected the cells from oxidation. In this study, it had been shown that paracetamol could be metabolized by yeast cell suspension or cell free extracellular protein, reflecting possibility of role of enzyme that can not be separated from cell and that is soluble, which is common phenomenon in mammalian cell system. Paracetamol of 3 mg/ml increased lipid peroxidation, a marker of oxidative stress. A green tea polyphenol, (+)-catechin of 0.1 mg/ml did not decrease lipid peroxidation content induced by paracetamol. At higher concentration (2 mg/ml), solely (+)-catechin did not increase lipid peroxidation content. Paracetamol or (+)-catechin induced slightly activity of superoxide dismutase enzyme. The data indicated that paracetamol metabolism or toxicity in the yeast may be similar to that of mammalian cells. In this condition, it suggested that (+)-catechin is one of polyphenol green tea that has weak activity of antioxidant and consequently has weak activity of prooxidant. Mechanism of paracetamol toxicity in C. tropicalis is still to be studied with emphasis on the free radical formation and drug metabolism.
SIFAT PROTEKSI EKSTRAK AIR PANAS TEH {Camellia sinensis (L.) Kuntze} HIJAU PADA KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARACETAMOL Heddy Julistiono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1943

Abstract

In order to develop yeast Candida tropicalis as a model cell for evaluation of substance having anti- or pro-oxidant activity in cell level, the effect of hot water tea {Camellia sinensis (LJ Kuntze) extract on peroxidized lipids, a marker of oxidative stress and cell mortality in the yeast caused by paracetamol has been evaluated. Incubation of yeast cell in the presence of 1.38 % green tea for 2 h decreased cell viability followed with increasing of peroxidized lipids, whereas 0.27 % green tea caused increasing of peroxidized lipids without causing cell death.Yeast cell was not affected by 0.1 % green tea. Incubation of yeast cell with presence of 0.15 % (g/v) paracetamol for 2 h did not cause cell mortality, however content of peroxidized lipids increased significantly. In the presence of higher dose of paracetamol (0.3 %) cell viability remarkably decreased and followed with increasing of peroxidized lipids significantly. Green tea of 0.1 % increased cell viability of cells treated with 0.3 % paracetamol while peroxidised lipids decreased. The data indicated that high dose of paracetamol caused oxidative stress in yeast cells, while green tea with lower concentration might protect paracetamol toxicity due to its antioxidant property. Although the antioxidant property, high dose of green tea could cause oxidation due to its pro-oxidant activity. In conclusion, yeast C. tropicalis could be potentially used as a model cell to evaluate substances having antioxidant property in cell level.
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