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All Journal BERITA BIOLOGI
Ismu Purnaningsih
Indonesian Culture Collection (InaCC), National Research and Innovation Agency (BRIN)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ANALISIS MORFOLOGI DAN FILOGENETIK MOLEKULER ALGA HIJAU COCCOID YANG DIISOLASI DARI PULAU ENGGANO Debora Christin Purbani; Diah Radini Noerdjito; Ismu Purnaningsih; Yeni Yuliani; Danang Ambar Prabowo
BERITA BIOLOGI Vol 21, No 3 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4215

Abstract

A combined of morphological characters and molecular approach, is important for evaluating the current classification of microalgae. Phylogenetic trees based on 18S rDNA gene sequence analysis provide useful tools to distinguish between inter- and intra-specific morphologically similar species of coccoid green algae. The aim of the study was to compare the morphological characters and conduct molecular analysis of coccoid green algae isolated from Enggano Island, located southwest of Sumatera, Indonesia. Coccoid green algae samples were collected from different sites at Enggano Island by using a simple random sampling. The single algae were isolated and transferred to IMK medium in flasks by using microcapillaries method. The morphological characteristics of green algae were observed under culture conditions using light microscopy and the phylogenetic positions of the isolated strains were defined according to the 18S rDNA gene sequences. According to homology search (BLAST) and phylogenetic tree analysis, four of the isolated coccoid green algae possessed high sequence similarity, ranging between 90-100%, to Chlorellavulgaris, Auxenochlorella protothecoides, Miractinium reisseri, and Micractinium balenophorum.
Co-Authors Danang Ambar Prabowo Debora Christin Purbani Diah Radini Noerdjito Yeni Yuliani