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Yuyu Suryasari Poerba
Pusat Penelitian Biologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENYIMPANAN SERBUKSARI PISANG LIAR Musa acuminata Colla UNTUK MENDUKUNG PROGRAM PEMULIAAN PISANG BUDIDAYA Erlin Rachman; Yuyu Suryasari Poerba; Fajaruddin Ahmad
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.486

Abstract

Research on pollen storage of four varieties and one subspecies of wild banana, Musa acuminata Colla, was carried out to support genetic improvement of banana cultivars through hybridization. The four wild varieties, breviformis, zebrina, malaccensis, bantamensis and a subspecies banksii cultivated at Cibinong Science Center (CSC-LIPI), Cibinong. Anthers were collected, put on bread paper,dried on hot plate at 40ºC for about two hours, open dried at room temperature in the laboratory (about 26ºC) for overnight, prepared for storage in two ways: -using and, -without silica gel, then stored in three storage temperatures, -5ºC, 5ºC and room temperature. Pollen germination was observed one day before storage, one week, four or five weeks and 12 weeks of storage. At the beginning, M. acuminata ssp banksii had highest pollen germination percentage, followed respectively by varieties bantamensis, breviformis, malaccensis and zebrina. The pollen germination ranks for the five banana varieties were remaining constant up to 12 weeks of storage though pollen germination dynamics occurred week by week. Silica gel may be recommended to be used until 5 weeks of storage. Storage temperature of -5ºC and 5ºC may be recommended as better storage temperature compared to room temperature. No special pattern shown by pollen tube vigor, but subspecies banksii pollen stored without silica gel, at 5ºC and room temperature tends to form better pollen tube vigor than stored at -5ºC.
CHROMOSOME COUNT ON YOUNG ANTHER OF BANANA MALE BUD USING EZYMATIC MACERATION AND DAPI STAINING IN SLIDE PREPARATION Fajarudin Ahmad; Yuyu Suryasari Poerba
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3851

Abstract

Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting. 
STUDI KERAGAMAN GENETIK PULAI [Alstonia scholaris (L.) R.Br.] BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA Yuyu Suryasari Poerba
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i5.1900

Abstract

Alstonia scholaris (L.) R.Br. is a popular timber and medicinal tree species in Indonesia.The species is valued for its quality light wood timber and for its medicinal properties.Information on its existing genetic potential is currently lacking. The present study was carried out to optimize PCR and to screen primers among accessions collected from different part of region in Indonesia using random amplified polymorphic DNA (RAPD) markers in order to suggest appropriate primer and PCR conditions used in A.scholaris. Results showed that 26 primers generated 575 scorable bands of which 524 (92 %) were polymorphic. Fourteen highly polymorphic primers (100% polymorphic) are recorded from 48 primer used, i.e.OPA-2, OPA-03, OPA-05, OPA-06, OPA-10,OPA-12, OPA-15, OPA-18, OPA-19, OPC-03, OPC-10, OPC-12, OPC-17, and OPN-14. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method.The range of genetic distance was from 0.18-0.45.The molecular dara grouped the genotypes into three main clusters.
Co-Authors Erlin Rachman Fajaruddin Ahmad Fajarudin Ahmad