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All Journal BERITA BIOLOGI Jurnal Ilmu Kefarmasian Indonesia
Yoice Srikandace
Pusat Penelitian Bioteknologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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IDENTIFIKASI MOLEKULAR ISOLAT KAPANG PENGHASIL ß-GLUCAN BERDASARKAN DAERAH INTERNAL TRANSCRIBED SPACER (ITS) Yoice Srikandace; Ines Irene Caterina A; Wibowo Mangunwardoyo
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1987

Abstract

Research was conducted to identify the fungal isolate G.23 which produces b-glucan from the Biopharmacy Laboratorium's collection.Indonesian Institute of Sciences (LIP1) based on the sequence of the Internal Transcribed Spacer (ITS) regions.DNA was isolated from mycelia and the ITS region was amplified by Polymerase Chain Reaction (PCR) with ITS1 and ITS4 primers. The PCR product was purified using the QIAquick PCR Purification kit (Qiagen). BigDye terminator cycle sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystem) was used and the product was purified with the AutoSEQ G-50 Kit (Qiagen).The sequence obtained analysed using Basic Local Alignment Search Tool nucleotide (BLAST)n homology search.The BLASTn result showed that the fungal isolate G.23 belongs to the genus Aspergillus. Taxa closely related to this isolate were Aspergillus elegans,A. ochraceus and A.sclerotiorum with 96% sequence homology.ClustalX was used for sequence-alignment. Phylogenetic analysis was constructed using the Neighbour Joining (NJ) method with Kimura two parameters. The phylogenetic tree obtained showed that fungal isolate G.23 separated from A. elegans, A. ochraceus and A. sclerotiorum which indicated that fungal G.23 belonged to a different spesies.Morphological observation on culture and microscopic appearance of the fungal isolate G.23 supported that this isolate differs from A. elegans, A. ochraceus and A. sclerotiorum.
Seleksi Mikroba Endofit Curcuma Zedoria dalam Memproduksi Senyawa Kimia Antimikroba Yoice Srikandace; Yatri Hapsari; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 5 No 2 (2007): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.699 KB)

Abstract

It is well known, that temu putih (Curcuma zedoaria Berg.), a rhizome of the Zingiberaceae, is oftenly used as medicine. In this connection, the potential secondary metabolites as anticancer and antimicrobes are produced by endophytic microbes in the plant. In this research, five endophytic microbes from the Curcuma zedoaria’s rhizome were fermented to produce compounds as antimicrobes.
Co-Authors Ines Irene Caterina A Partomuan Simanjuntak Wibowo Mangunwardoyo Yatri Hapsari