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All Journal BERITA BIOLOGI
Adi Santoso
Pusat Penelitian Bioteknologi - LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PERKEMBANGAN TEKNOLOGI SEL MAMALIA CHINESE HAMSTER OVARY (CHO) UNTUK PRODUKSI OBAT BERBASIS PROTEIN Adi Santoso
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3705

Abstract

Chinese hamsters ovary (CHO) and its derivative such as CHO-DXB11 cells, CHO-K1, CHO-DG44 and CHO-S are mammalian cells that are often used for production of therapeutic protein drugs. The CHO cells often used for protein production have several advantages including 1) host cells that are safe to use in drug production, 2) the level of production of proteins produced can be increased by amplifying genes using methotrexate (MTX), 3) having the capacity to make post-translation modificationsand 4) CHO cells can be adapted to grow in suspension. The high need for protein-based drugs triggers the development of basic knowledge and innovation in production of recombinant proteins. The impressive technological advances in CHO cell technology have made these cells can be used to produce proteins around 10 g/liter in order to meet the market demand. The first protein successfully produced using CHO mammalian cells was the therapeutic Tissue Plasminogen Activator (r-tPA, Activase) protein used for stroke patients. The presence of this drug is quickly followed by several other types of drugs. In this review, history of development of CHO cells, the contribution of CHO cells to basic research, progress of effective line cell screening and development technology are discussed.
GLIKOBIOLOGI, GLIKANS DAN GLIKOPROTEIN BESERTA APLIKASINYA DALAM KESEHATAN Adi Santoso
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3991

Abstract

Glycobiology is a study of the structure, biosynthesis, glycosylation and biology of glycans that are widespread in nature. Through the process of glycosylation which is one of the most post-translational forms of protein modification, macromolecular structures that are as diverse as glycoproteins can be formed. In other words, glycosylation is one of the most common structural modifications used by biological systems to expand proteomic diversity. This makes glycosylation a very high prevalence, estimated at 50-70% of all proteins are glycoproteins. Glycosylation can affect proteolysis patterns, ligand-receptor interactions, oncogenic signal transduction, body immunity, cell adhesion and cell matrix. Because of the high level of structural variability that arises from the glycosylation process, many new strategies can be made using the uniqueness of this glycoprotein modification, especially in the pharmaceutical field. This includes modifications in protein engineering in the expression systems of yeast, plant cells and mammalian cells. 
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