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Elidar Naiola
Bidang Mikrobiologi, Pusat Penelitian Biologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PEMBUATAN STARTER UNTUK EKSTRAKSI MINYAK KELAPA MURNI MENGGUNAKAN MIKROBA AMILOLITIK Elidar Naiola
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.801

Abstract

The thirteen isolates of amylolitic microbes had been tested their ability to extract the oil from " coconut milk" and nine of them could break the emulsion and separated the oil from the water and protein. The aim of this study was to find a starter that can be used for producing the coconut oil by using molase and "gula aer" gewang (Corypha utan Lamk.) palm sugar as the substrates.The result suggest that by using the isolates (ferm. 1 and ferm.2), "gula aer"gewang can be used as a substrate without supplemented by organic nitrogen. The starter prepared with isolate ferm. 1 containing cells of microbe about 10.2 x 10 cell/ml and prepared with ferm.2 about 9.0 - 10.2 x 10 cell/ml. After 4 weeks the amount of the cells decreased to 0.98 x 10 cell/ml and 0.90 x 10cell/ml, respectively, The amount of microbes were stable until 12 weeks.The starter conducted the fermentation processes at 40°C for 16 hours and produced the coconut oil. The extracted oil content about 85% saturated fatty acids and 42% of them was lauric acid. Another chemical component of the extracted oil were Iodine numbers, peroxide numbers and free fatty acid (FFA), they were 5.98%, 2.51 Meq/kg and 0.41%, respectively.
AKTIVITAS ANTIMIKROBA FLAVONOID - GLIKOSIDA HASIL SINTESIS SECARA TRANSGLIKOSILASI ENZIMATIK Yati Sudaryati Soeka; Elidar Naiola; Joko Sulistyo
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.825

Abstract

Flavonoid-glycoside was synthesized enzymatically using CGT-ase (EC.2.4.1.19) of indigenous Bacillus licheniformis in a phosphate buffer pH 6.0 at 45°C for 24 h, through transglycosylation reaction in the present of flavonoid those were extracted from rhizomes such as ginger, flngerroot, turmeric, white turmeric and white curcuma as natural acceptors, and commercial rice,cassava, corn and wheat flour as substrates.The result showed that CGT-ase of B. licheniformis transferred a glycosyl moiety in a bilayer enzymatic reaction system of n-hexanol and phosphate buffer yielding glycosides as transfer products in the present of wheat flour as substrate and white curcuma extract as its acceptor.An inhibitory effects of the synthesized flavonoid glycosides against microbial growth was furthermore examined. It was found that flavonoid-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200 ppm on the growth of Bacillus subtilis, Escherichia coli and Saccharomyces cerevisiae, however no effect when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains.
KHARAKTERISASI ENZIM KASAR GLUKOAMILASE DARI Saccharomycopsis sp. Elidar Naiola
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i3.795

Abstract

Saccharomycopsis sp. produced glucoamylase (a-1,4 glucanglucohydrolase; EC 3.2.1.3) shown by the presence of clear zone on media containing 0.2% yeast extract, 0.5% pepton, 0.3% KH2PO4, 0.05% MgSO4. 7H2O, 0.01% CaClr2H2O, 2% agar and 2% soluble starch. In liquid media containing soluble starch glucoamylase production reached a maximum at 2 days fermentation, with levels of 4.2 x 10 U/ml (one unit activity is define as micromoles of glucose produce per ml per minute).Studies on the glucoamylase characterization revealed that the optimum temperature for activity was 40°C - 50°C. The enzyme was stable for lh at 45"C - 55°C, while at 60°C to 65°C, 40% of the original activities were lost. The optimum pH of the enzyme was 6.0. After incubation of crude enzyme solution for 1 h at pH 8.0, a decrease of about 40% of its original activity was observed. Saccharomycopsis sp. produced high glucoamylase when it was grown in media containing rice flour as carbon source with the glucoamylase activity at 9,28 x 10 U/ml.
Co-Authors Joko Sulistyo Yati Sudaryati Soeka